2023 2024 Student Forum > Management Forum > Main Forum

 
  #2  
26th August 2015, 02:19 PM
Super Moderator
 
Join Date: May 2012
Re: Birla Institute of Technology Mesra - Master of Technology in Biotechnology

As you want to get the syllabus of Birla Institute of Technology Mesra - Master of Technology in Biotechnology so here is the information of the same for you:


Syllabus of Birla Institute of Technology Mesra - Master of Technology in Biotechnology:
Semester 1:
TBT 1001: Bioprocess Engineering:
Module I
Principles of enzyme catalysis:

Introduction to enzymes, mechanistic models for simple enzyme kinetics, rate parameters, models for more complex enzyme kinetics, effect of pH and temperature, methods of immobilization, diffusional limitations in immobilized enzyme systems, brief introduction to large scale enzyme production.

Module II
Microbial growth:

Introduction to metabolism; Nutrient transport; Glycolysis; TCA cycle and other pathways; Control of metabolism; Factors affecting microbial growth; Stoichiometry: mass balances; Stoichiometry: energy balances; Growth kinetics; Measurement of growth.

Module III
Bioreactors:

Introduction to bioreactors; Batch and Fed-batch bioreactors, Continuous bioreactors; Immobilized cells; Bioreactor operation; Sterilization; Aeration; Sensors; Instrumentation; Culture-specific design aspects: plant/mammalian cell culture reactors.

Module IV
Agitation and aeration:

Types of impellors and sparger, oxygen transfer rate, oxygen uptake rate, volumetric oxygen transfer rate (kLa), measurement of kLa, power requirement for agitation in gaseous and non gaseous systems.

Module V
Scale up, operation and control of bioreactors:

Concepts of various bioreactor configurations, scale-up, various criteria for scale-up, scale-down, bioreactor instrumentation and control.

Module VI
Industrial Microbiology:

Production of microbial polysaccharides, microbial transformation of steroids and sterols: Bioprocess technology, beer brewing, cheese manufacture, mold–modified foods, Wine, Vinegar, Microbial production of amino acids, antibiotics, microbial enzymes, organic acids; Strain improvement, culture preservation and inoculum development. probiotics. Production of antibiotics, biopolymers, vaccines.

Module VII
Industrial Processes and Process economics:

Description of industrial processes; Process flow sheeting; Process economics

Books Recommended:

1. Lee, Biochemical Engineering
2. Shuler and Kargi, Bioprocess Engineering – Basic Concepts. Prentice Hall PTR
3. Aiba and Humphary. Biochemical Engineering
4. Doran, Bioprocess Engineering Principles
5. Bailey and Ollis, Biochemical Engineering Fundamentals

Texts/References:

1. Michael Shuler and Fikret Kargi, Bioprocess Engineering: Basic Concepts, 2nd Edition, Prentice Hall, Englewood Cliffs, NJ, 2002.
2. Pauline Doran, Bioprocess engineering principles, 1 Edition, Academic Press, 1995.
3. Colin Ratledge, Bjorn Kristiansen, Basic Biotechnology, 2nd Edition, Cambridge University Press, 2001.
4. Roger Harrison et al., Bioseparations Science and Engineering, Oxford University Press,
2003.

TBT 1003: Bioprocess Engineering:
Module I
Basics Concepts:

DNA Structure and properties; Restriction and DNA modifying enzymes; Cohesive and blunt end ligation; Linkers; Adaptors; Homopolymeric tailing; Labeling of DNA using different techniques; Hybridization techniques: Southern, Northern, Colony and Fluorescence and in situ hybridization; Chromatin Immunoprecipitation; DNA-Protein Interactions-Electromobility shift assay; DNaseI footprinting; Methyl interference assay

Module II
Cloning Vectors:

Plasmids; Bacteriophages; M13 mp vectors; Phagemids; Insertion and Replacement vectors; EMBL; Shuttle vectors; Cosmids; Artificial chromosome vectors (YACs; BACs); Animal Virus derived vectors-SV-40; vaccinia/bacculo & retroviral vectors; Expression vectors (eukaryotic and prokaryotic); Protein purification; His-tag; GST-tag; MBP-tag etc.; Intein-based vectors; Inclusion bodies; Methodologies to reduce formation of inclusion bodies; Plant based vectors, Ti and Ri as vectors, Yeast vectors

Module III
Cloning Methodologies:

Insertion of Foreign DNA into Host Cells; Transformation; Construction of libraries; Isolation of mRNA and total RNA; cDNA and genomic libraries; cDNA and genomic cloning; Metagenomics and its role in environment, Expression cloning; Jumping and hopping libraries; Southwestern and Farwestern cloning; Protein-protein interactive cloning and Yeast two hybrid system; Phage display; Principles in maximizing gene expression; Transgenics; cDNA and intragenic arrays; Differential gene expression and protein array, Introduction of DNA into mammalian cells; Transfection techniques

Module IV
PCR and Its Applications:

Primer design; Fidelity of thermostable enzymes; DNA polymerases; Types of PCR – multiplex, nested, reverse transcriptase, real time PCR, touchdown PCR, hot start PCR, colony PCR, cloning of PCR products; T-vectors; PCR in gene recombination; Deletion; addition; Overlap extension; and SOEing; PCR in molecular diagnostics; Viral and bacterial load detection; PCR based mutagenesis, Mutation detection: SSCP, DGGE, RFLP, Oligo Ligation Assay (OLA), MCC (Mismatch Chemical Cleavage, ASA (Allele-Specific Amplification), PTT (Protein Truncation Test)

Module V
Sequencing methods:

Enzymatic DNA sequencing; Chemical sequencing of DNA; Automated DNA sequencing; Next generation DNA sequencing techniques, RNA sequencing; Chemical Synthesis of oligonucleotides;

Module VI
Gene silencing techniques:

Small double stranded RNAs; siRNA technology; Micro RNA; Construction of siRNA vectors; Principle and application of gene silencing;

Module VII
Gene knockouts and Gene Therapy:

Creation of knock out C. elegans, Arabidopsis and mice; Disease model; Somatic and germ-line therapy- in vivo and ex-vivo; Suicide gene therapy; Gene replacement; Gene targeting;

Text/References:

1. S.B. Primrose, R.M. Twyman and R.W.Old; Principles of Gene Manipulation. 6th Edition, S.B.University Press, 2001.
2. J. Sambrook and D.W. Russel; Molecular Cloning: A Laboratory Manual, Vols 1-3, CSHL, 2001.
3. Brown TA, Genomes, 3rd ed. Garland Science 2006
4. Selected papers from scientific journals.
5. Technical Literature from Stratagene, Promega,

TBT 1005: Biophysics:
Module I
Biophysical basics:

Structure of Atom, Schrodinger’s theory, Quantum numbers, Pauli’s exclusion principle, Heisenberg uncertainty principle, Vander waals forces, Molecular orbital theories, Electro chemical cell, Brownian Motion.

Module II
Hydrodynamic and Bimolecular spectroscopy I:

Centrifugation & Ultracentrifugation, Viscometry, Osmosis, Diffusion and Surface tension, Principle, Instrument Design, Methods & Applications of UV-Visible Spectra, IR Spectra, Raman Spectra, Fluorescence spectra, NMR and ESR Spectra., Linear and Circular Dichroism, Nuclear Magnetic Resonance Spectroscopy.

Module III
Diffraction Techniques and Bimolecular spectroscopy II
:
Crystals, Molecular crystal symmetry, Bragg’s Law X ray diffraction by crystals. Principle, Instrument Design, Methods & Applications of Polarimetry, Refractometry, Flowcytometry, Cytophotometry, Raman spectroscopy, vibrational spectroscopy in biology, Mossbauer spectroscopy. Mass Spectrometry for Protein Identification.

Module IV
Microscopy:

Compound, Phasecontrast, Fluorescence, Scanning & Transmission Electron Microscopy, CCD Camera, Introduction to Atomic Force Microscopy

Module V
Membrane Biophysics:

Relation between membrane potential & cell characteristics, Zeta, Stern & total electrochemical potential, Helmholtz-Smoluchowski equation, Transmembranes potential & it’s measurement by microelectrodes. Role of carriers in ion transport (ex: -Valinomycin & gramicidin), Transporting ATPase-Na-K ATPase.

Module VI
Neurobiophysics and Applications of Radioactivity:

Synaptic transmission, Physicochemical basis of membrane potential, Resting and action potential, Propagation of action potential, Voltage clamp and patch-clamp techniques, Autoradiography, General principles, Types & constitutions of photographic emulsion.

Module VII
Electrophysiology and Nuclear Medicine:

Principles of Electrocardiography, Heart- an electric potential sources, ECG waveforms, Standard lead systems, ECG preamplifiers, ECG readout devices, ECG machine, Measurements, Principles of Electroencephalography, Basic principles of Nuclear Medicine, Diagnostic use of Radioisotopes In-vivo & In-vitro procedures, (Single isotope, Double isotope methods).

For more detailed information I am uploading a PDF file which is free to download:

(Syllabus of Birla Institute of Technology Mesra - Master of Technology in Biotechnology)

https://www.bitmesra.ac.in/UploadedD...ogy%20Prog.pdf

Contact Details:
Birla Institute of Technology, Mesra
Mesra
Ranchi,
Jharkhand 835215
India


[MAP]Birla Institute of Technology, Mesra Jharkhand[/MAP]


Quick Reply
Your Username: Click here to log in

Message:
Options

Thread Tools Search this Thread



All times are GMT +5. The time now is 05:05 AM.


Powered by vBulletin® Version 3.8.11
Copyright ©2000 - 2024, vBulletin Solutions Inc.
SEO by vBSEO 3.6.0 PL2

1 2 3 4