Mysore University Biotechnology syllabus

Hii sir, I wants to Phd in Biotechnology from Mysore University will you please provide me the syllabus of the Phd Entrance Exam for Biotechnology ?

[pawan]

The University of Mysore is a public state university in Mysore, Karnataka, India

Syllabus of the Phd Entrance Exam Biotechnology was as follow


Unit 1: Biochemistry and Biophysics
Composition, structure, conformation and function of biomolecules -carbohydrates, lipids, proteins, nucleic acids and vitamins.

Principles of biophysical chemistry (pH, buffer, reaction kinetics).
Bioenergetics, glycolysis, oxidative phosphorylation, coupled reaction, group transfer, biological energy transducers.

Principles of catalysis and enzymes, enzyme kinetics, enzyme regulation, inhibition, isozymes. Conformation of proteins (Ramachandran plot, secondary structure, domains, motif and folds).

Unit 2: Methods in Biology
Centrifugation Techniques Chromatographic Techniques- General principles, TLC, column chromatography, HPLC,

Adsorption chromatography, Partition chromatography, Ion exchange chromatography, Exclusion chromatography, GLC, Affinity chromatography.
Electrophoretic Techniques: General principles, Native gels, SDS-PAGE, IEF, 2D gel electrophoresis, Agarose gel electrophoresis, Pulse field gel electrophoresis, Capillary electrophoresis.

Spectroscopic techniques: UV/visible, fluorescence, circular dichroism, NMR, ESR spectroscopy, X-ray diffraction, mass spectrometry.
Radiolabeling techniques: Detection and measurement of radioisotopes, molecular imaging of radioactive material, safety guidelines.

Microscopic techniques: Light microscopy, scanning and transmission electron microscopy, fluorescent and confocal microscopy.

Statistical Methods: Measures of central tendency and dispersion; probability distributions (Binomial, Poisson and normal); Sampling distribution; parametric and non-parametric statistics;
Confidence Interval; Errors; Levels of significance; Regression and Correlation; t-test; Analysis of variance and multiple range tests, chi-square test, experimental design, data transformation

Unit 3: Microbiology
Historical perspectives; Pure culture techniques.
General outline and classification of viruses, fungi, bacteria and molecular taxonomy.
Archaea: Halophiles, methanogens, thermophiles.
Microbial growth: Growth curve, measurement of growth, continuous culture, factors affecting growth; culture collection and maintenance of cultures.
Microbial nutrition and metabolism: Metabolic diversity among microorganisms;
chemolithotrophy; hydrogen-iron-nitrite-oxidizing bacteria; methanogenesis; fermentation. Microbes and environment: Nutrient cycles; quorum sensing; biofuels; prebiotics and probiotics.
Microbial diseases: Tuberculosis, AIDS, candiasis, malaria.
Important diseases of plants: Downy mildew of pearl millet, panama wilt of banana, bacterial leaf blight of rice, TMV.
Antibiotics: Types, mode of action and resistance.

Unit 4: Cell Biology
Membrane structure and function: Structure of fluid mosaic model of membrane, lipid bilayer,

transport across membrane, mechanism of sorting and regulation of intracellular transport. Structural organization and function of intracellular organelles.
Organization of chromosomes: Structure of chromatin and nucleosome, heterochromatin, euchromatin.

Cell division and cell cycle: Mitosis and meiosis, cell cycle and regulation.
Cell signaling: Peptide and steroid hormones and their receptors, signal transduction pathways, secondary messengers, regulation of signaling pathways.
Cellular communication: Cytoskeletal elements, cell adhesion molecules, extracellular matrix, neurotransmission and its regulation.

Cancer: Oncogenes, tumor suppressor genes, cancer and cell cycle, virus-induced cancer, metastasis, interaction of cancer cells with normal cells, apoptosis, chemotherapy.


Unit 5: Molecular Biology and Genetics
DNA replication, repair and recombination in prokaryotes and eukaryotes: Mechanism of replications, enzymes, fidelity of replication, DNA damage and repair mechanisms, homologous and site-specific recombination.

RNA synthesis and processing in prokaryotes and eukaryotes: Transcription factors and machinery, formation of initiation complex, transcription activator and repressor, RNA polymerases, capping, elongation, and termination, RNA processing, RNA editing, splicing and polyadenylation.

Protein synthesis and processing in prokaryotes and eukaryotes: Ribosome, formation of initiation complex, initiation factors, elongation, termination, genetic code, aminoacylation of tRNA, translational inhibitors, Post-translational modification of proteins.

Control of gene expression at transcription and translation level: regulating the expression of prokaryotic and eukaryotic genes, role of chromatin in gene expression, DNA methylation, gene silencing.


Rest of the syllabus you may get from the below Attachement that is Free to Download


For any query you may contact to the Mysore university the contact details are given below :

Contact details :
Mysore university
Address: Crawfordhall, Krishnaraja Bouleward Road, Mysuru, Karnataka 570006
Phone: 0821 241 9450