JKPSC Biotechnology

I have completed M.SC Degree in Biotechnology Discipline. I got information from somewhere about recruitment in JKPSC for the post of Lecturer for Biotechnology. I am thinking to apply for this recruitment, but before applying I want to see syllabus. So pls provide detailed syllabus.

[sumit]

As you are looking for syllabus of Biotechnology for recruitment for the post of Lecturer in JKPSC, so here I am providing complete syllabus:

JKPSC Recruitment Syllabus for Lecturer in Biotechnology
1. Cellular diversity – An overview.

2. Structure of Prokaryotic and Eukaryotic cells.

3. Cellular organelles-Plasma membrane, cell wall, Mitochondria, Chloroplast,
Nucleus and other organelles; their structural organization.

4. Transport of nutrients, ions and macromolecules across membranes, endocytosis,
phagocytosis & pinocytosis, Vesicular transport & secretory pathways.

5. Cellular energy transaction-Role of mitochondria and chloroplast.

6. Cell cycle-molecular events and regulatory controls, cell cycle checkpoint with
emphasis on animal cells & yeast cell divisions.

7. Cancer: Types, causes, molecular basis of cancer cell behaviour.

8. Chemical foundation of Biology-pH, pK, acids, bases, buffers, weak bonds
(hydrogen bonding, hydrophobic, hydrophilic, Vander waals bonding and ionic
interactions) covalent bonds, Water: physical properties and structure of water.

9. Principles of thermodynamics-concept of free energy, Gibbs Helmoltz equation,
free energy and equilibrium constants, free energy and electrode (Cell) potential.

10. Carbohydrates – Definition, Nomenclature, Classification, Occurrence,
characteristics and Biological significance. Structure and conformation of sugars.
Stereoisomerism and optical isomerism. Chemical reactions of functional groups
present in carbohydrates, Amino sugars. Structure and function of homo-andhetero-
polysaccharides. Mucopolysaccharides and Proteoglycans. Types of
gylcoproteins-N and O linked glycosylations. Polysaccharides-Types, structural
features, methods for compositional analysis.

11. Proteins: structure and conformation of proteins. Secondary structures. Prediction
of secondary structure of proteins. Ramachandran plots. Tertiary structure of
proteins and forces stabilizing the tertiary structure. Mechanism of protein folding
– Chaperons.

12. Fatty Acids: Definition, nomenclature and properties. Saturated and unsaturated
fatty acids. Essential fatty acids. Classification of lipids. Chemical composition of
fats, Properties of glycerides

13. Pure culture techniques, theory & practice of sterilization, growth curve,
synchronous growth, continuous culture, Growth as effected by environmental
factors like Temperature, Acidity, Alkalinity, water availability and oxygen,
culture collection and maintenance of cultures.

14. Prokaryotic cells: structure function. Cell wall of eubacteria (Peptidoglycan) and
related molecules; Outer membrance of Gram negative bacteria; cell wall and cell
membrance synthesis; Flagella and motility; cell Inclusions like endospores, gas
vesicles.

15. Transformation, conjugation, Transduction, Recombination, Plasmids and
Transposons. Bacterial genetic maps with reference to E-Coli. Brief introduction to
life cycle and molecular biology of some important pathogens; of Hepatitis,
Tuberculosis, HIV.

16. Carbohydrate Metabolism: Glycolysis, TCA cycle, pentose phosphate pathway,
Glucuronate pathway, Gluconeogensis. Glycolysis, TCA cycle, pentose phosphate
pathway, Gluconeogensis. Glycogen metabolism and its regulation. Hormonal
control of carbohydrate metabolism.

17. Basic principles of centrifugation, types of centrifugation, differential
centrifugation, density gradient centrifugation, materials used for making density
gradient.

18. Basic principles & types of electrophoresis, Agarose gel electrophoresis, PAGE,
SDS-PAGE and isoelectric focussing.

19. Blotting techniques like Southern and Western blotting & their applications in
molecular biology.

20. Brief description & tabulation of data & its graphical representation.

21. Measures of central tendency & dispersion; mean, median, mode, range, standard
deviation, variance. Idea of two types of errors and level of significance, test of
significance (F& t-test); chisquare tests.

22. Simple linear regression and correlation.

23. Introduction to computers; Organization; low level & high-level languages; binary
number systems. Introduction to MS-Office (word Processing, spreadsheets &
presentation software).

24. Introduction to Internet & its applications.

25. Replication of DNA: direction and methods of replication, replication in
prokaryotes, chromosomal replication, Topoisomerases-I and II in replication.
Replication forks, Eukaryotes.

26. Messenger RNA biosynthesis in prokaryotes-RNA Polymerase & promoter,
specificity, initiation. Elongation & termination of transcription. Transcriptional
repression & de-repression, operon concept (Lac).

27. Eukaryotic transcription, RNA polymerase, general & specific transcription
factors, regulatory elements & mechanism of transcription regulation, RNA
processing & stability.

28. Prokaryotic & Eukaryotic translation, the translational machinery, mechanism of
initiation, elongation & termination, regulation of translation, Co- and posttranslational
modification of proteins. Degeneracy of genetic code, wobble
hypothesis Genetic code in Mitochondria.