Indian Council of Agricultural Research syllabus of M.Sc Plant Biotechnology

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PRINCIPLES OF BIOTECHNOLOGY 2+1
Objective
To familiarize the students with the fundamental principles of
Biotechnology, various developments in Biotechnology and its potential
applications.

Theory
UNIT I

History, scope and importance; DNA structure, function and metabolism.

UNIT II
DNA modifying enzymes and vectors; Methods of recombinant DNA
technology; Nucleic acid hybridization; Gene libraries; PCR amplification;
Plant and animal cell and tissue culture techniques and their applications.

UNIT III
Molecular markers and their applications; DNA sequencing; Applications
of gene cloning in basic and applied research; Genetic engineering and
transgenics; Genomics, transcriptomics and proteomics.

UNIT IV
General application of biotechnology in Agriculture, Medicine, Animal
husbandry, Environmental remediation, Energy production and Forensics;
Public perception of biotechnology; Bio-safety and bioethics issues;
Intellectual property rights in biotechnology.

Practical
i. Isolation of genomic and plasmid DNA
ii. Gel electrophoresis techniques
iii. Restriction enzyme digestion, ligation, transformation and screening of
transformants
iv. PCR and molecular marker analysis
v. Plant tissue culture: media preparation, cell and explant culture,
regeneration and transformation.

Suggested Readings
Becker JM, Coldwell GA & Zachgo EA. 2007. Biotechnology - a
Laboratory Course. Academic Press.
Brown CM, Campbell I & Priest FG. 2005. Introduction to Biotechnology.
Panima Pub.
Brown TA. Gene Cloning and DNA Analysis. 5th Ed. Blackwell Publishing.
Dale JW & von Schantz M. 2002. From Genes to Genomes: Concepts and
Applications of DNA Technology. John Wiley & Sons.
Gupta PK. 2004. Biotechnology and Genomics. Rastogi Publications.
Sambrook J, Fritsch T & Maniatis T. 2001. Molecular Cloning - a
Laboratory Manual. 2nd Ed. Cold Spring Harbour Laboratory Press.
Singh BD. 2007. Biotechnology Expanding Horiozon. Kalyani Publishers.







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Contact Details:
Indian Council of Agricultural Research
Krishi Bhavan,
Dr. Rajendra Prasad Road,
New Delhi,
Delhi 110001 ‎
011 2584 3277 ‎
India

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