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25th September 2014, 10:17 AM
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Join Date: Apr 2013
Re: BIS Scientist Biochemistry Exam Syllabus

Here I am providing the Syllabus for BIS Scientist Biochemistry Exam which you are looking for .

Written examination is of 2½ hrs duration
The question paper will be of two parts with a total of 120 objective type questions with multiple choice answers.

Part I - consists of 80 questions on respective technical discipline.

Part II - consists of 40 questions on aptitude/analytical reasoning including English.

The 40 questions from PART-II consists of

--> General Aptitude,
--> Verbal and Non-Verbal Reasoning
--> English
--> General Knowledge

Biochemistry
Organization of life. Importance of water. Cell structure and organelles. Structure and function of biomolecules: Amino acids, Carbohydrates, Lipids, Proteins and Nucleic acids. Biochemical separation techniques and characterization: ion exchange, size exclusion and affinity chromatography, electrophoresis, UV-visible, fluorescence and Mass spectrometry. Protein structure, folding and function: Myoglobin, Hemoglobin, Lysozyme, Ribonuclease A, Carboxypeptidase and Chymotrypsin. Enzyme kinetics including its regulation and inhibition, Vitamins and Coenzymes.
Metabolism and bioenergetics. Generation and utilization of ATP. Metabolic pathways and their regulation: glycolysis, TCA cycle, pentose phosphate pathway, oxidative phosphorylation, gluconeogenesis, glycogen and fatty acid metabolism. Metabolism of Nitrogen containing compounds: nitrogen fixation, amino acids and nucleotides. Photosynthesis: the Calvin cycle.
Biological membranes. Transport across membranes. Signal transduction; hormones and neurotransmitters.
DNA replication, transcription and translation. Biochemical regulation of gene expression. Recombinant DNA technology and applications: PCR, site directed mutagenesis and DNA-microarray.
Immune system. Active and passive immunity. Complement system. Antibody structure, function and diversity. Cells of the immune system: T, B and macrophages. T and B cell activation. Major histocompatibilty complex. T cell receptor. Immunological techniques: Immunodiffusion, immunoelectrophoresis, RIA and ELISA.


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