M Sc Biotechnology in Allahabad University

Can you tell me in detail about the M Sc Biotechnology course offered in Allahabad University as I am planning to take admission in it?

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The Centre of Biotechnology was established in 2000 under Institute of Inter Disciplinary Studies (IIDS) of Allahabad University, supported by Department of Biotechnology, Govt. of India, New Delhi. It offers Masters Programme (4 semesters) in Biotechnology as well as D. Phil. programme.

M Sc Biotechnology in Allahabad University

For Masters Programme students are admitted through all India Combined Entrance Examination conducted by Jawaharlal Nehru University every year.

M Sc Biotechnology Syllabus

Semester I (20 credits)
Papers (12 credits)
Paper 1: Analytical Techniques (3 credits)
Paper 2: Biochemistry (4 credits)
Paper 3: Genetics and Microbiology (3 credits)
Paper 4: Biostatistics and Computer Applications in Biotechnology (2 credits)
Laboratory Technique I (8 credits)
Semester II (20 credits)
Papers (12 credits)
Paper 1: Biophysical Chemistry (4 credits)
Paper 2: Cell Biology (2 credits)
Paper 3: Molecular Biology (4 credits)
Paper 4: Fermentation Technology (2 credits)
Laboratory Technique II (8 credits)
Semester III (20 credits)
Papers (12 credits)
Paper 1: Animal Cell Biotechnology (2 credits)
Paper 2: Genetic Engineering (4 credits)
Paper 3: Immunology (4 credits)
Paper 4: Plant Biotechnology (2 credits)
Laboratory Technique III (8 credits)
Semester IV (20 credits)
Papers
Elective Papers (any two of the following) (2 credits each)
Elective 1: Proteomics
Elective 2: Environmental Biotechnology
Elective 3: Nanobiotechnology
Elective 4: Molecular Therapeutics
Project work (16 credits)
Grand total of credits = 80

M Sc Biotechnology Syllabus
Semester-I; Paper: 1
ANALYTICAL TECHNIQUES
(3 credits)
1. Basic techniques: Buffer preparations; pH measurement; Cell disintegration; Dialysis and ultra
filtration.
2. Spectroscopy: Principles and applications of UV-Visible, Fluorescence and Infra Red spectroscopy.
3. Chromatography: Principles and applications of Paper and Thin layer chromatography; Size
exclusion, Ion exchange, Hydrophobic, Reverse phase and Affinity chromatography; HPLC and FPLC.
4. Electrophoresis:
Theory and application of Polyacrylamide and Agarose gel electrophoresis; Different variants of
polyacrylamide gel electrophoresis (PAGE) like native, SDS-PAGE, 2D-PAGE, Blotting Techniques:
Southern, Western and Northern blotting, Immunoblotting, Immunoelectrophoresis,
Immunofluorescence, ELISA.
5. Centrifugation: Sedimentation, Analytical ultra centrifugation, Preparative ultra centrifugation: zonal
and equilibrium density gradient ultracentrifugation
6. Radioactivity: Concept of radioactivity; Radioactivity counting methods with principles of different
types of counters; Autoradiography; Applications of radioactive tracers in biology.
7. Microscopy: Principles and applications of Simple, Compound and Phase contrast microscope,
Fluorescence microscope, confocal microscope, Time lapse imaging, Electron microscopy: SEM &
TEM, Cryo-Electron microscopy
Text books/References:
1. Principles and Techniques of Practical Biochemistry by Keith Wilson and John Walker (5th
edition, 2000).
2. Physical Biochemistry, application to Biochemistry and Molecular Biology by David Freifelder
(2nd edition, 1982).
3. Analytical Biochemistry by D. Holme and H. Peck (3rd edition, 1998).
4. Protein Purification: Principles and Practice by Robert K. Scopes (3rd edition, 1993).
Semester-I; Paper: 2
BIOCHEMISTRY
(4 credits)
1. Chemical foundations of Biology: Composition of living matter, Water-properties, pH, pKa, acids,
bases, buffers; weak bonds, covalent bonds.
2. Protein: physical and chemical properties of amino acids; Primary, secondary, tertiary and
quaternary structure; Globular and fibrous proteins; Amino acid composition and primary structure
analysis, Structure-function relationship in model proteins like ribonuclease A, myoglobin and
hemoglobin.
3. Carbohydrates: mono, di and polysaccharides; Structural and functional role; Glycoprotein and
Glycolipid.
4. Lipids: Structure and properties of storage and membrane lipids; Lipoproteins; Structural
organization of biological membrane.
5. Nucleic acids: Structure and properties of purines, pyrimidines, nucleosides, nucleotides, helical
structure of DNA. Different forms of DNA. Denaturation and renaturation of DNA.
6. Enzyme catalysis: General principles of catalysis; Quantitation of enzyme activity and efficiency;
Enzyme characterization and Michaelis-Menten kinetics; Relevance of enzymes in metabolic regulation,
activation, inhibition and covalent modification; single substrate enzymes.
7. Metabolic pathways: Energy concepts and energy rich compounds; Glycolysis, gluconeogenesis,
pentose phosphate pathway, citric acid cycle and oxidative phosphorylation; Fatty acid biosynthesis and
oxidation.
8. Vitamins: Structure and biological properties
Text books/References:
1. Biochemistry by Jeremy M. Berg, John L. Tymoczko and Lubert Stryer (6th edition, 2008).
2. Lehninger Principles of Biochemistry by David L. Nelson and Michael M. Cox (5th edition,
2009).
3. Biochemistry by V.Voet and J.G. Voet (4th edition Dec 2010).
Semester-I; Paper: 3
GENETICS AND MICROBIOLOGY
(3 credits)
1. Mendelian genetics: Laws of dominance, segregation & independent assortment; Incomplete
dominance, complementary genes, epistasis, lethal genes, duplicate genes, and multiple allelism.
Extrachromosomal inheritance: mitochondrial & chloroplast inheritance. Linkage & crossing over; sex
linkage & sex determination, Population & Evolutionary genetics; Gene Mapping
2. Microbial gene transfer mechanisms: conjugation, transduction, transformation: genetic systems of
Neurospora, Yeast, E. coli, Arabidopsis and Drosophila
3. Bacterial mutants & mutation: Isolation, Useful phenotypes (auxotrophic, conditional, lethal,
resistant); Mutation rate, Types of mutations (base pair changes; frameshift; insertion; deletion; tandem
duplication), Reversion vs suppression; Mutagenic agents; Molecular mechanism of mutations, practical
applications of mutations; Assay of mutagenic agents (Ames test); variations in chromosome number
and structure, DNA repair mechanisms
4. Study of microorganisms: General characteristics and salient features related to structure, function,
physiology and significance of cyanobacteria, actinomycetes, fungi, yeast, viruses, rickettsia &
mycoplasma. Ultrastructure of a bacterial cell: spore, cell wall, flagella, cell membrane, capsule, pili.
Microbial growth: Batch, fed-batch, continuous kinetics, synchronous growth, yield constants, methods
of growth estimation, stringent response, death of a bacterial cell.
5. Microbial systematics, Molecular Taxonomy, Basic microbiological techniques: Microscopy, Pure
culture, nutrition, enrichment, sterilization, disinfection, safety in the microbiological laboratory.
6. Study of ecophysiological, biochemical and nutritional aspects of phylogenetically diverse
representative groups of organisms: extremophiles - thermophiles, psychrofiles, halophiles,
methanogens, archaebacteria, Nitrogen fixing organisms and nitrogen fixing genes, Mycorrhiza: types
and its functions
7. Microbial Ecology: interactions among microbial populations, microbial interaction with animals,
microbial interaction with plants
8. Diseases of humans: Bacterial meningitis, Botulism, Poliomyletis, African trypanosomiasis,
salmonellosis, giardiasis, hepatitis and AIDS.
9. Antibiotics: types & mode of action, resistance to antibiotics
Text books/References:
1) Pleczar MJ Jr., Chan ECS and Kreig NR., Microbiology, 5th Edition, 1993
2) Lansing Prescott, John Harley and Donald Klein, Microbiology
3) R.Y.Stanier, General Microbiology
4) D. Peter Snustad, Michael J. Simmons, John B. Jenkins, PRINCIPLES OF GENETICS

Contact Detail:
University Of Allahabad
University Road, Old Katra, Allahabad, Uttar Pradesh 211002 ‎

Map:
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