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My brother has applied for recruitment for the Post of Scientific Officer (DNA) in StateForensic Science Laboratory HP conducted by Himachal Pradesh Public Service Commission. So will you please provide syllabus of this recruitment Exam of Himachal Pradesh Public Service Commission?

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As you are looking for syllabus of recruitment for the Post of Scientific Officer (DNA) in StateForensic Science Laboratory HP conducted by Himachal Pradesh Public Service Commission, so here I am providing detailed syllabus:

Himachal Pradesh Public Service Commission Recruitment Scientific Officer (DNA) in State Forensic Science Laboratory HP Syllabus:
History and Development of Forensic Science, Definition of Forensic Science, Need ofForensic Science, Basic Principles of Forensic Science (Law of Individuality, Principle ofexchange, law of probability). Biological evidence: Importance, nature, location, collection and evaluation. Forensic significance of body fluids as blood, semen, saliva,sweat, milk and urine, their collection and identification. Preparation of solutions,buffers, use of buffers in DNA analysis. Concept of Normality, Molarity, Molality andtheir calculations.

Electrophoresis Theory and General Principles, Various factors affectingelectrophoresis, Low and High Voltage electrophoresis, Horizontal and VerticalElectrophoresis. Various electrophoresis techniques- Immuno-electrophoresis, NativePAGE, Sodium dodecyl sulphate (SDS-PAGE) polyacrylamide gel electrophoresis, Isoelectricfocusing (IEF), Capillary electrophoresis-Theory and basic principles,Instrumentation, Forensic applications.

DNA Profiling: Introduction, History of DNA Typing, human genetics, mitotosis,meiosis, heredity, alleles, mutations and population genetics, Hardy Weinberg law,molecular biology of DNA, variations, polymorphism, RFLP analysis, PCRamplifications, VNTRs, STRs, Mitochondrial DNA, SNPs,

Sampling in forensic DNA analysis, DNA, Multiplex PCR, STR Markers used in Forensic
DNA analysis, Commercially available STR Kits, Stutter products, non-templateaddition, microvariants, null alleles and mutation rates, issue of degraded DNA, PCRinhibition, contamination, mixed samples and low copy number. Use of SNPs andother bi-allelic markers in forensic DNA analysis. Y-STR based testing. Non-humanDNA testing and microbial forensics.

Use of Capillary electrophoresis in forensic DNA profiling, validated capillaryelectrophoresis platforms for forensic DNA, fluorescent labeling in multiplex kits,fluorescent detection. Instrumentation for forensic STR typinf, ABI310,3100,3130,3500 etc. STR genotyping issue.

Evaluation of results, frequency estimate calculations, interpretations, allelefrequency determination, match probability, DNA data bases CODIS, GenBank etc. STRpopulation data base analysis, likelihood ratios, source attribution, analysis mixedDNA profiles, kinship and parentage testing, probability of paternity etc. DNA analysisin mass disasters.

General Knowledge

General Knowledge of H.P.