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  #1  
11th October 2012, 09:59 AM
koundinya
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TET Gene PBR322

I have no any knowledge about TET gene pbr322 please provide me that site address where I can get sufficient information about it so mail me this site
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  #2  
13th October 2012, 11:39 AM
Super Moderator
 
Join Date: May 2012
Re: TET Gene PBR322

PBR322 is a plasmid and was the first widely-used E. coli cloning vectors. Created in 1977 in the laboratory of Herbert Boyer at the University of California San Francisco, it was named after the Mexican postdoctoral researchers who constructed it.

The p stands for "plasmid and BR for "Bolivar" and "Rodriguez."

PBR322 is 4361 base pairs in length and contains the replicon of plasmid pMB1, the ampR gene, encoding the ampicillin resistance protein (source plasmid RSF2124) and the tetR gene, encoding the tetracycline confrontation protein (source plasmid pSC101).


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