TBP for RGPV

Hi I would like to have the information about the course syllabus of the subject which is offered by the RGPV which includes the TATA binding proteins (TBP) and TBP associated factors (TAF)?

[sumit]

The course syllabus of the subject which is offered by the RGPV which includes the TATA binding proteins (TBP) and TBP associated factors (TAF) is M.Tech Biotechnology 1st Semester (Grading System) ant the subject is Cell and Molecular Biology.

The Detailed Course syllabus for the Cell and Molecular Biology is as follows:

Module-I
Cell cycle and Genome Organization
Genome Organization in prokaryotes and eukaryotes - DNA content and C-value paradox - methods to measure DNA content variation - Various types of DNA sequences – simple sequences, repetitive sequences, Junk DNA or selfish DNA, tandem gene clusters, satellites Variety of DNA structures: double helix, Z-DNA, B-DNA, Mechanism of DNA replication: prokaryotes and eukaryotes, Overview of the cell cycle, Factors involved in cell cycle, Mitosis, Meiosis, cell cycle control, cell check points.

Module –II
Replication and Cell Signaling Mechanisms
DNA replication models, mode of action, DNA damage, DNA repair and recombination, Organization structures and function of ribonucleoproteins; Photoreactivation; Nucleotide excision repair; Mismatch correction; SOS repair; Recombination: Homologous and non- homologous; Site specific recombination; Chi sequences in prokaryotes; Gene targeting; Gene disruption; Signal transduction, primary and secondary messengers, involvement of G proteins, protein kinases, serine, threonine and tyrosine kinases, mechanism of signaling by steroids, cell death.

Module – III
Prokaryotic & Eukaryotic Transcription
Prokaryotic Transcription; Regulation of transcription, Termination-Rho-dependent and independent, Attenuation; Transcriptional regulation-Positive and negative; Operon concept-lac, trp, ara, his, and gal operons; Transcriptional control in lambda phage; Anti-termination, Transcript processing; Processing of tRNA and rRNA Eukaryotic transcription and regulation; RNA polymerase structure and assembly; RNA polymerase I, II, III; Eukaryotic promoters and enhancers; General Transcription factors; TATA binding proteins (TBP) and TBP associated factors (TAF); Activators and repressors; Transcriptional and posttranscriptional gene silencing

Module – IV
Post Transcriptional Modifications Processing of hnRNA, tRNA, rRNA; 5'-Cap formation; 3'-end processing and polyadenylation; Splicing; RNA editing; Nuclear export of mRNA; mRNA stability; Catalytic RNA.
Translation & Transport Translation machinery; Ribosomes; Composition and assembly; Universal genetic code; Degeneracy of codons; Termination codons; Isoaccepting tRNA; Wobble hypothesis; Mechanism of initiation, elongation and termination; Co- and post-translational modifications; Genetic code in mitochondria; Transport of proteins and molecular chaperones; Protein stability; Protein turnover and degradation.

Module – V
Diverse type of oncogenesis
Viral and cellular oncogenes; Tumor suppressor genes from humans; Structure, function and mechanism of action of pRB and p53 tumor suppressor proteins; Activation of oncogenes and dominant negative effect; Suppression of tumor suppressor genes; DNA virus/ cell immortalization ,Oncogenes as transcriptional activators. Strategies of chemotherapy and gene therapy against cancer; translating therapies from laboratory to clinic; Gene discovery in cancer research, Mechanisms of diverse type of cancers.
Case studies.

Lab on Cell & Molecular Biology
1. Isolation and Quantitation of cellular macromolecules (DNA, RNA and Protein) .
2. cDNA Synthesis
3. Transformation and Preparation of competent cells.
4. Antibiotics sensitivity test on microbial cultures
5. Agarose gel electrophoresis of DNA fragments.
6. SDS PAGE for resolution of proteins.
7. Elution of DNA from an agarose gel.
8. Preparation of metaphase Chromosome.
9. Karyotyping and banding Pattern (G-banding).
10. Preparations of blood smear for study of sex chromatin.