Model Paper for ARS/NET Exam

I need ARS NET exam Previous years Question paper for better preparation, will yiu please provide here?

[Arun Vats]

You are looking for ARS NET exam previous year question paper, here i am giving:

1. Which of the following pairs are anomers?

a) Ribose and Ribulose
b) Glucose and mannose
c) Pyruvate lactate
d) α-D-glucose and β-D-glucose

2. Hybridoma technology is used in the production of

a) mRNA
b) Monoclonal serum
c) Monoclonal antibodies
d) Polyclonal antibodies

3. For mitochondrial structure and function mt-DNA specifies

a) 10%
b) 20%
c) 30%
d) 40%

4. Which of the following reagent is useful for visualizing DNA?

a) Uracil
b) DNA polymerase
c) Diphenylamine
d) Ethidium bromide

5. Homologous pairs line up along equatorial plane during

a) Anaphase II
b) Metaphase I
c) Telophase
d) Interphase

6. Between mitotic divisions, the cell is in

a) G0
b) G1
c) G2
d) S phase

7. Most plant cells are surrounded by a rigid cell wall made primarily of

a) Monosaccharides
b) Pectin
c) Chitins
d) Polysaccharides

8. The organelle that functions in the breakdown of cells and tissues is the

a) Episomes
b) Mitochondrion
c) Centrosomes
d) Lysosomes

9. Endoplasmic reticulam

a) Is found only in animals
b) is a system of membrane bound channels
c) is called rough if mitochondria is attached to it
d) is a site of ATP production

10. “9+2” describes the basic structure of which of the following one

a) chromosome
b) Basal body
c) Chloroplast
d) Flagellum

11. The function of nucleus includes

a) Cellular respiration
b) Synthesis of proteins
c) Housing the hereditary information
d) Synthesis of carbohydrates

12. Bacculoviruses

a) RNA viruses
b) DNA viruses
c) Both a and b
d) None of the above

13. Secondary metabolites like antibiotics are not essential for

a) Log phase growth
b) Exponential growth
c) Both a and b
d) None of these

14. The restriction enzymes useful to the molecular biologist belongs to

a) Type I
b) Type II
c) Type III
d) Type IV

15. Which of the following vector is suitable for DNA sequencing

a) EMBL
b)PBR 322
c) M 13
d) Lambda

16. During which process, the free end of the chromosome moves from Hfr donor in to the F cell across an intracellular bridge called piles

a) Transformation
b) Transduction
c) Conjugation
d) All of these

17. Francis Jacob and Monod Proposed

a) Lock and Key Hypothesis
b)Gene for Protein Synthesis
c) Operon Synthesis
d) Gene for protein synthesis

18. State which of the following is not a desired characteristic of a vector?

a) Unique restriction site
b) Large Size
c) Gene that confer antibiotic resistance
d) Autonomous replication

19. Which of the following is a carrier hosts for new genes?

a) EcoRI
b) Bacteriophages
c) Nucleus
d) Transplanted Organs

20. Which of the following chemical is used for preparation of competent cell?

a) HCl
b) NaCl
c) CaCl2
d) Glycine

Answers
1. d) α-D-glucose and β-D-glucose
2. c) Monoclonal antibodies
3. a) 10%
4. d) Ethidium bromide
5. b) Metaphase I
6. b) G1
7. d) Poly saccharides
8. d) Lysosomes
9. b) is a system of membrane bound channels
10. d) Flagellum
11. c) Housing the hereditary information
12. b)DNA viruses
13. c) Both a and b
14. b) Type II
15. c) M 13
16. c) Conjugation
17. c) Operon Synthesis
18. b) Large Size
19. b) Bacteriophages
20. c) CaCl2

Can you provide me some questions from the question paper of Model Paper for ARS/NET (Agricultural Research Service/ National Eligibility Test) Exam ?

[Quick Sam]

Some questions from the question paper of Model Paper for ARS/NET (Agricultural Research Service/ National Eligibility Test) Exam are as follows:

1. Answer the following questions briefly

i) What advantage RILs have over DHs in genetic mapping
ii) How an F2 population can be immortalized for dissecting both additive and dominance variation
iii) How MgCl2 concentration affect the efficiency of PCR
iv) What is stuttering and why it happened?
v) During DNA isolation , the temperature of extraction buffer is
maintained at 650C. Why?
vi) Why do we need larger population size for fine mapping of a gene?
vii) What will happen to PCR, if the template DNA concentration is >200 ng.
viii) While constructing a cDNA library, why do we need to isolate RNA from different plant parts and under different stimuli?
ix) Why the nucleotide sequence of 3’ end of forward and reverse primers should not be complementary
x) What will happen if the nutrient medium used for the tissue culture is not supplemented with glycine?
xi) Why is it necessary to culture embryos invitro in intergeneric crosses?
xii) Will there be any limitation for releasing glyphosate (herbicide) resistant transgenic rice in Eastern India?
2. What is T-DNA? Differentiate between T-DNA of Ti and Ri plasmids. Discuss the mechanism of T-DNA transfer to plant cells.

3. What is genetic code? Is genetic code universal? Discuss it with suitable examples?

4. Enlist various approaches used for cloning of genes in eukaryotes. Describe the procedure for map based cloning? What limitation he map based cloning approach has over other methods. Name any five genes that have been cloned in crop plants using map based cloning approach.

5. What is antisense RNA technology? How is it useful in plant improvement? Discuss it with suitable examples?

6. What is binary vector? Discuss the structure and function of binary vector? How it is useful in plant improvement?

7. What are haploids? Discuss various approaches used for the production of haploids in crop plants? What limitations each of these methods have? What uses haploids have in basic genetic studies and in crop improvement?

8. a) What are transgenic crops? Discuss two major approaches used for production of transgenics. What advantages and limitation each of these approaches have over each other?
b) Area under commercial transgenic crops has increased continuously since the year 2000. Still a large number of NGOs are opposing commercialization of transgenics. What are the major concerns for the opposition? Give your view point for or against each of these concerns?

9. What do you understand by nif genes? Now that the genomes of both nitrogen fixing bacteria (Rhizhobium) and legumes (soybean, Pigeon pea) are sequenced, how do you see the possibility of converting the non nitrogen fixing cereals like rice and wheat into nitrogen fixing system?

10. a) What are SSR markers? Enumerate the procedure for how SSR markers are being developed? What advantage SSR markers over RFLP markers?
b) Enlist any four approaches used for labelling? Describe the procedure for ‘hexamer primer’ labelling of nucleic acids?

11. What are cDNA libraries? Describe the procedure to develop cDNA libraries. What advantage cDNA libraries would have over genomic DNA libraries?

12. What are QTLs? Write down the procedure to map a QTL? How NILs are used for mapping / fine mapping of a QTL. Name any five economically important QTLs so far cloned from crop plants?

13. What is marker assisted selection and how its differs from markers assisted back crossing? If you have the responsibility of pyramiding four genes from four different source into one elite line, which approach will you follow and why?

14. a)Write different steps and enzyme involved in citric acid cycle.
b) How do enzymes catalyze chemical reaction with in a cell? What is Km and how can an enzyme be characterized by this parameter?

15. Promoters are organ specific and extremely important for regulating the expression if a gene. You are given the responsibility of cloning tapetum specific promoter(s) from rice. Write down the procedure in detail?

16. What are gene libraries? Discuss the utility of genomic libraries in functional and structural genomics.

17. What are the merits and demerits of different technique for introducing foreign DNA into plant cells? Discuss the structural organization of the Ti plasmid and T-DNA.

18. Compare and contrast between followings:-
a) Prokaryotic vs. Eukaryotic Ribosomes
b) Southern vs.Northern hybridisation
c) Chromosome walking vs.Chromosome jumping
d) Gene tagging vs.Genome mapping

19. After genetic transformation, how the cells carrying the recombinant vectors are selected? Enlist advantages and disadvantages of various selection methods. Give an analytical account, why in many cases, even if the transgenic is obtained in the transgenic plants?

20. What is RNAi technology? What are the possible applications of this technology in agriculture and crop improvement? How it differs from post transcriptional gene silencing?

21. What is genomics? What do you understand “complete genome sequencing”? What is the advantage of using BAC in such sequencing programmes? Give a brief account of any two plant sequencing projects in India.

22. What is the microarray technique? How can it be used to differentiate a normal plant from diseased plant? Discuss the application of this application s of this technique in plant biology.

23. A) What are the genetic engineering strategies to create the following traits in transgenic crops- a) abiotic stress b) virus resistance?

B) What is ‘golden rice’?How was it developed?

24. What are the requirements for establishing a plant tissue culture laboratory? Write a note on the composition and preparation methods of plant tissue culture medium for transgenic crops.
25. What is restriction modification system? Give a detailed account of restriction systems in bacteria .Why are they so important in recombinant DNA technology?

26. Discuss various potential and realized applications of genetic engineering in crop improvement. Give suitable examples to justify the statements.

27. Define ‘allele mining’. How is it helpful for crop improvement? Write strategies for ‘allele mining’.

28. What is QTL mapping? How it is different from associate mapping? How candidate gene and Germplasm sets are selected for associate mapping?

29. What is biodiesel? Which crops are potential source of biodiesel? How production potential of these crops can be increased by using biotechnological tools?

30. What are molecular probes? How can these prepared and labelled? Discuss their utility.

31. Outline the events involved in translation of mRNA into polypeptide in prokaryotes?

32. Write short notes on the following:
i) Site directed mutagenesisii) Frame shift mutationsiii)Suppressor mutationsiv) Base analogs
33. Explain different types of nucleic acid hybridization and their applications in molecular biology.

34. Discuss the different methods of production of haploids in plants with their relative merits and demerits. Discuss use of haploids in plant breeding and gene mapping in comparison to conventional methods.

35. What is RNA splicing? How is it different from RNA editing?