Kashmir University M.Sc. Biochemistry 2nd Sem BCH507 MOLECULAR BIOLOGY Syllabus

[Arun Vats]

Kashmir University M.Sc. Biochemistry 2nd Sem BCH507 MOLECULAR BIOLOGY Syllabus

SEMESTER:2

PAPER 2 (BCH- 507) BIOENERGETICS AND INTERMEDIARY

METABOLISM UNIT-I Bioenergetics Energy transformation by biological systems Concept and significance of free energy Phosphoryl transfer potential Coupled reactions ATP as energy currency Redox, electrochemical and membrane potentials Oxidative phosphorylation Structural organization of respiratory chain Metabolite transport across mitochondrial membrane Electron flow in respiratory chain Coupling of oxidation and phosphorylation Structure and function of ATP-synthesis complex Short-circuiting of proton gradient Regulation of rate of oxidative phosphorylation Toxic oxygen metabolites, their scavenging and interactions with biomolecules

UNIT-II Intermediary metabolism Approach for studying metabolism Carbohydrates metabolism Glycolysis Citric acid cycle, its function in energy generation and biosynthesis of energy rich bonds Pentose phosphate pathway and its regulation Alternate pathways of carbohydrate metabolism

SEMESTER:2 Gluconeogenesis Interconversions of sugars Biosynthesis of glycogen, starch and oligosaccharides Regulation of blood glucose homeostasis Hormonal regulation of carbohydrates metabolism

UNIT-III Lipids metabolism Fatty acids biosynthesis Acetyl CoA carboxylase Fatty acids synthesis Desaturase and elongase Fatty acids oxidation α, β, ω, oxidation and lipo-oxidation. Lipid biosynthesis Biosynthesis of triacylglycerols Phosphoglycerides and sphingolipids Biosynthetic pathways for terpenes Steroids and prostaglandins Ketone bodies Formation and utilization Metabolism of circulatory lipids Chylomicrons, LDL, HDL and VLDL

UNIT-IV Amino acids metabolism Biosynthesis and degradation of amino acids and their regulation Specific aspects of amino acid metabolism Urea cycle and its regulation In-born errors of amino acids metabolism


SEMESTER:2 Nucleic Acids metabolism Biosynthesis or purines and pyrimidines Regulation of purine and pyrimidine biosynthesis Degradation of purines and pyrimidine Structure and regulation of ribonucleotide reductase Biosynthesis of ribonucleotides, deoxy ribonucleotides and polynucleotide Inhibitors of nucleic acid biosynthesis