#1
10th May 2016, 08:06 AM
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HCL UV Cutoff
What is the HCL UV Cutoff for common mobile phases for that will you please get the information so that I can have idea about it?
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#2
10th May 2016, 08:54 AM
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Re: HCL UV Cutoff
I will help you here to get the details about HCL UV Cutoff for common mobile phases so that you can check it easily. Here is the cut off Solvent UV Cutoffs Solvent UV Cutoffs Acetonitrile 190 nm Amyl alcohol 210 nm Acetone 330 nm Amyl chloride 225 nm Benzene 280 nm Cyclohexane 200 nm Cyclopentane 200 nm Carbon disulfide 380 nm Carbon tetracholoride 265 nm Chloroform 245 nm Diethyl amine 275 nm Ethanol 210 nm Ethylene glycol 210 nm 2-Butoxyethanol 220 nm p-Dioxane 215 nm Ethyl acetate 256 nm Ethylene dichloride 230 nm Ethyl ether 220 nm Ethyl sulfide 290 nm Isopropyl ether 220 nm Isopropyl chloride 225 nm Isopropanol 205 nm Methanol 205 nm Methylene chloride 233 nm Methyl-isobutyl ketone 334 nm Methyl ethyl ketone 330 nm 1-Nitropropane 380 nm Nitromethane 380 nm Octane 215 nm n-Pentane 190 nm Petroleum ehter 210 nm n-Propanol 210 nm n-Propyl chloride 225 nm Pyridine 330 nm Toluene 285 nm Tetrahydrofuran 230 nm Xylene 290 nm Wavelength Cutoffs for Common Mobile Phases Mobile Phase UV Cutoff 1% Acetic acid 230 nm 10 mM Ammonium bicarbonate 190 nm 0.1% BRIJ 35 190 nm 0.1% CHAPS 215 nm 50 mM Diammonium phosphate 230 nm 10 mM Ammonium acetate 205 nm 1 mM Na2-EDTA 190 nm 0.1% HCl 190 nm 10 mM HEPES (pH 7.6) 225 nm 10 mM MES (pH 6.0) 225 nm 10 mM Potassium phosphate-monobasic 190 nm 10 mM Potassium phosphate-dibasic 230 nm PIC Reagent B-6 (1 vial/liter) 225 nm PIC Reagent D-4 (1 vial/liter) 190 nm PIC Reagent A (1 vial/liter) < 200 nm 1 M NaCl 208 nm 10 mM Sodium citrate 225 nm 10 mM Sodium acetate 205 nm 0.1% SDS 190 nm 10 mM Sodium formate 200 nm 0.1% Trifluoroacetic acid (TFA) 205 nm 0.1% Triethylamine 235 nm 0.15 Tween 20 190 nm 0.1% Triton-X 100 240 nm 20 mM Tris-HCl (pH 7.0) 204 nm 20 mM Tris-HCl (pH 8.0) 212 nm |