The M.Sc. (Applied Microbiology) Syllabus of CCSU (Chaudhary Charan Singh University), Meerut on which the question paper is based is as follows:
Course 1, Code- AM 101: Instrumentation and Microbial Techniques
Unit I: Microscopy & Staining techniques: Basic principles for the examination of microbes by light, dark field, phase contrast, confocal, fluorescent and electron (transmission and scanning) microscopy; Micrometry; Specimen preparation and basic principles of Simple, Gram’s stain, Capsule, Endospore, Flagella, Acid fast and Nuclear/Geimsa’s staining.
Unit II: Basic principles and methods of sterilization: control of microorganisms by physical
methods: heat, filtration and radiation; chemical methods: phenolics, alcohols, halogens, heavy metals, quartenary ammonium compounds, aldehydes and sterilizing gases; evaluation of antimicrobial agent effectiveness. Principle and functioning of LAF.
Unit III: Basic principles and methods of media preparation: types of culture media: simple media, complex media, synthetic media, enriched media, selective media, indicator media, differential media, anaerobic media; pH and buffers; Pure culture techniques: streak plate, dilution plate and spread plate method; maintenance of pure cultures; methods of preservation of various microbes.
Unit IV: Basic principles and applications of spectrophotometry & Chromatography : Beer-Lambert law; interaction of radiation with matter, absorption of radiation, emission of radiation; UV-Vis spectrophotometry, Fluorimetry, Flame photometry and atomic absorption spectrophotometry; Chromatography (paper, thin layer, column, gel filtration, ion-exchange and affinity chromatography); GLC, HPLC and FPLC.
Unit V: Miscellaneous techniques : Principles and applications of Electrophoresis for protein and DNA; Iso-electric focusing and 2-D gel electrophoresis; Autoradiography, X-Ray diffraction; Centrifugation; Ultracentifugation; Dialysis, Ultrafiltration; Lyophilization.
Suggested Readings (Latest Editions):
1.Nelson D and Cox MM. (2010). Lehninger’s Principles of Biochemistry. W.H. Freeman and Company, New York.
2.Wilson K. and Walker J. (2013). Principles and Techniques of Biochemistry and Molecular
Biology. Cambridge University Press.
3. Willey J, Sherwood L. and Woolverton C (2014). Prescott’s Microbiology, 9th edi McGraw Hill.
4. Upadhyaya and Nath (2015) Biophysical chemistry, Himalaya pub. House.
5. T.A.Brown (2016). Gene cloning and DNA analysis, an introduction,Wiley Blackwell pub.
6. B.D.Singh (2015). Biotechnology, Kalyani publication.
M.Sc. (Applied Microbiology) Syllabus CCSU (Chaudhary Charan Singh University) 
