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  #1  
2nd June 2015, 10:34 AM
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Calcutta University Botany Question Papers

Provide me the previous year question papers of M Sc Botany of Calcutta University as my exams are near by and so need it for preparation of the exam to score good marks? Provide me the syllabus of M Sc Botany of Calcutta University in PDF format?

The syllabus of M.Sc. (Master in Science) Botany program offered by University of Calcutta on which the question paper is based is as follows:

FIRST SEMESTER



Bot C11: Microbiology: Theory (40 marks; 2.5 credits, 40 Lecture hours)

Section A (GENERAL MICROBIOLOGY)


Microbial taxonomy: Basis of bacterial classification: analysis of phenetic, genetic and phylogenetic
characteristics; polyphasic approaches to bacterial taxonomy. Salient features of major bacterial groups
according to Bergeys Manual of Systematic Bacteriology.
Methods in microbiology: Types of culture media, isolation of pure cultures, enrichment culture techniques,
maintenance and preservation of bacterial cultures. Control of microorganisms: physical and chemical
methods.
Growth and differentiation: Measurement of growth, growth kinetics, synchronous growth, and continuous
culture. Physico-chemical factors influencing bacterial growth. Differentiation: sporogenesis- physiobiochemical and genetic aspects, Caulobacter differentiation.

Section B (MICROBIAL PHYSIOLOGY)


Photosynthesis and fermentative metabolism: Bacterial photosynthesis (anoxygenic and oxygenic): antennae
complex and reaction centre, chemosynthesis. Bacterial fermentative pathways: lactic acid, propionic acid,
mixed and butanol fermentation.
Biosynthetic pathways: Biosynthesis of peptidoglycan and extracellular polysaccharides and polyesters.
Nitrogen metabolism: ammonification, nitrification, denitrification and nitrogen fixation. Nif genes: functions
and regulation. Metabolism of amino acids: amino acid biosynthesis pathways: aspartate, and glutamate family,
regulation and overproduction.


Section C (MICROBIAL GENETICS)


Genetic analysis of bacteria: Conjugation: molecular mechanism of gene transfer and regulation. Conjugation
mapping, Plasmids: types, function and application. Transformation: Natural transformation and competence;
molecular mechanism of transformation. Transduction: Generalized and specialized transduction-T4, T7 and
lambda phages. Lysogenic phages: genome organization and its regulation.
Transposons: Types of bacterial transposons. Detection, regulation and molecular mechanism of transposition
in bacteria.
Gene regulation: Positive and negative gene regulation and attenuation, lac, gal, trp, and araoperons and their
applications. Quorum sensing.CRISPR-CAS discovery, mode of action and application.

Section D (APPLIED MICROBIOLOGY)


Environmental microbiology: Distribution and implications of microbes in air and water, waste water
treatment systems; Microbial production of pesticides; degradation of xenobiotics Bioremediation of heavy
metals.
Medical microbiology: Human microbiome, host pathogen interaction, pathogenicity of bacteria invasiveness
and toxigenicity, genetics of bacterial virulence, constitutive and inducible host defense mechanism, acquired
immunity and immune systems.
Chemotherapy: Principles of chemotherapy, general mode of action of various chemotherapeutic agents: Sulfa
drugs, antibiotics- classification and mode of action. antibiotic resistance, vaccines and antivirals.

Section E (VIROLOGY)


General Virology: Virus classification. General properties and ultra-structure.Viral genome
organizations.Cultivation of viruses, methods for detection and assay. Virus related agents: viriods, prions and
prion hypothesis.
Bacteriophages: General classification and properties, genome organization. Phage therapy and typing.
Genetic analysis of phages complementation and recombination tests with phages.
Plant viruses: Tobacco mosaic virus: capsid assembly, genome organization and replication.

PRACTICALS


1. Basic microbiological techniques: preparation of media, sterilization, slant and stab preparation. Pouring of
plates and pure culture by streak and pour plate method.
2. Qualitative and quantitative analysis of soil or water microbiota, characterization of selected pure culture
isolates by gram staining, simple and negative staining, endospore staining and physio-biochemical features
(extraceullar enzymes, antibiotic sensitivity/production).
3. Determination of bacterial growth and growth kinetics under batch cultivation (turbidimetric and cell count
method)
4. Bacterial transformation: Competent cell preparation, transformation, screening of transformed isolates.
5. Plasmid isolation and gel electrophoresis.
6. Bacteriophage titration, purification and quantification.
7. Isolation of antibiotic producing organism from soil and their sensitivity assay against standard laboratory
strains of bacteria.
8. Enrichment and isolation of nitrogen fixing bacteria from soil and their characterization.


SUGGESTED READINGS:


1. General Microbiology by R.Y. Stanier, JL Ingrahm, ML Wheelis and PR Painter.
2. Microbiology: Fundamentals and Applications by RM Atlas.
3. General Microbiology by HG Schlegel




Attached Files
File Type: pdf Calcutta University Botany Question Papers.pdf (1.07 MB, 367 views)

Last edited by sumit; 8th February 2020 at 03:28 PM.
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  #2  
13th November 2016, 11:36 PM
Niladri Sen
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Re: Calcutta University Botany Question Papers

I wanna get 10 years Botany honours question papers
  #3  
6th November 2019, 08:40 AM
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Re: Calcutta University Botany Question Papers

Can you provide me the syllabus of M.Sc. (Master in Science) –Botany program offered by University of Calcutta on which the question paper is based?


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