University of Pune Biochemistry

Can you provide me the Syllabus for M.Sc. Biochemistry (Credit System/Semester System) as offered by University of Pune as my exams are near by and I need it for preparation of it?

[pawan]

The Syllabus for M.Sc. Biochemistry (Credit System/Semester System) as offered by University of Pune is as follows:

SEMESTER I

BCH –170 Biomolecules

Biomolecules I: Carbohydrates and Lipids
1 The molecular logic of life: The chemical unity of diverse living organisms, composition of living matter. Macromolecules and their monomeric subunits.
2 Properties of Water: With interactions in aqueous systems. Ionization of water, weak acids and weak base. The pH scale, measurement of pH, pH metry, acid base titration curves. Buffers, biological buffer systems.
3 Carbohydrates: Classification, basic chemical structure, monosaccharides, aldoses, and ketoses, cyclic structure of monosaccharides, steroisomerism, anomers and epimers.
Sugar derivatives, deoxy sugars, amino sugars, and sugar acids. General reaction and properties.
4 Lipids: Classification, structure and function of major lipid subclasses-acylglycerols, circulating lipids, Separation techniques Lipoproteins, chylomicrons, LDL, HDL, and
VLDL. Pathological changes in lipid levels. Formation of micelles, monolayers, bilayer,
liposomes.
5 Vitamins and Co-enzymes: Classification, water-soluble and fat-soluble vitamins. Structure, dietary requirements, deficiency conditions, coenzyme forms.

Biomolecules II: Proteins
1. Amino acids: Classification, Properties, reactions, rare amino acids, and separation techniques.
2. Protein classification: Reactions, functions, properties peptide synthesis. Solid phase synthesis.
3. Structure:
a) Peptide bond, end group analysis, sequencing.
b) Secondary: X ray diffraction, alpha-helix bets- structure, β-helix, super secondary structure.
c) Tertiary Structure: Forces stabilizing, unfolding/ refolding expt. Prediction of tertiary Structure.
d) Quaternary structure – hemoglobin.
e) Ramachandran plot.
f) Helix coil transitions, Vander Walls, electrostatic, Hydrogen bonding, and hydrophobic interactions.
g) Energy terms in Biopolymer conformational calculation.

Syllabus for M.Sc. Biochemistry University of Pune