Sagar University Msc Biotech

I requires syllabus of MSc Biotechnology course of Sagar University . please provide here syllabus of MSc Biotechnology course of Sagar University in PDF file ?

[Arun Vats]

As you want I am here providing you syllabus of MSc Biotechnology course of Sagar University in PDF file.

MSc Biotechnology course:
Duration: 4 semesters
Total seats: 20

Syllabus :
Semester 1 :
Cell Biology
Bio-Molecules
Microbial Physiology and Genetics
Computer Applications and Biostatistics
Bioinformatics
Biofuel Technology
Scientific papers, presentation and writing skills

Semester 2:
Molecular biology
Macromolecules & Basic Enzymology
Biology of the Immune System
Biophysical chemistry
Instrumentation
Basic Virology
Proteomics

Semester 3:
Animal Biotechnology
Genetic Engineering
Bioprocess Engineering and technology
Plant Biotechnology
Genomics and Functional Genomics
Molecular Biology in Drug Discovery
Plant Tissue Culture

BIT C 121 Cell Biology
Theory
UNIT- I
1. Diversity of cell size and shape.
2. Cell theory.
3. Structure of Prokaryotic and Eukaryotic cell- isolation and growth of cells.
4. Microscopic Techniques for study of cells.

UNIT- II
1. Sub-cellular fractionation and criteria of functional integrity.
2. Cellular organelles-plasma membrane, cell wall, their structural organization.
3. Transport of nutrients, ions and macromolecules across membranes.
4. Cellular energy transactions-role of mitochondria and chloroplast.

UNIT- III
1. Cell cycle- molecular events and model systems.
2. Cellular responses to environmental signals in plants and animals- mechanisms of signal transduction.
3. Cell motility- cilia, flagella of eukaryotes and prokaryotes.
4. Biology of cancer.

UNIT- IV
1. Metabolite pathways and their regulation.
2. Biosynthesis of proteins in Eukaryotic cell, Co-and post-translational modification, intercellular protein traffic.
3. Cellular basis of differentiation and development- mitosis, gametogenesis and fertilization.
4. Development in drosophila and arabidopsis; spatial and temporary regulation of gene expression.

UNIT- V
1. Brief introduction to the life cycle and molecular biology of some important pathogens of AIDS, Malaria, Hepatitis.
2. Tuberculosis, Filaria, Kalazar.
3. Protein localization: synthesis of secretory and membrane proteins, import into nucleus, mitochondria, chloroplast and peroxisomes.
4. Receptor mediated endocytosis.

Practicals
1. Microscopy: bright field, phase contrast and fluorenscence microscopy.
2. Microtomy.
3. Instrumental methods for cell biology.
4. Sub cellular fractionation and marker enzymes.
5. Histochemical techniques.
6. Mitosis and Meiosis.

Books
1. Molecular Biology of Cell, Alberts, B. et.al.
2. Molecular Cell Biology, Lodish et.al.
3. Reproduction in Eukaryotic cells, DM Prescott, Academic Press.
4. Developmental Biology, SF Gilbert, Sinauer Associates Inc.
5. Cell in Development and Inheritance; EB Wilson, MacMilan, New York.
6. The Coiled Spring, Ethan Bier, Cold Spring Harbor Press.
7. Fertilization, FT Longo, Chapman and Hall.
8. Molecular Biology of Steroid and Nuclear Hormone Receptors, LP Freedman Birkhuser.

BIT C 122 Bio- Molecules
Theory
UNIT- I
1. pH, pK, acids, bases.
2. Buffers, weak bonds and covalent bonds.
3. Principles of thermodynamics.
4. Classes of organic compounds and functional groups- atomic and molecular dimensions, space filling and ball and stick models.

UNIT- II
1. Amino acids and peptides- classification, chemical reactions and physical properties.
2. Sugars- classification and reactions.
3. Heterocyclic compounds and secondary metabolites in living systems- nucleotides pigments and isoprenoides.
4. Proteins- classification and separation, purification and criteria of homogeneity, enc group analysis, hierarchy in structure and Ramchandran map.

UNIT- III
Separation techniques for different biomolecules.
1. Chromatography.
2. Electrophoresis.
3. Centrifugation.
4. 2 D and MALDI.
5.
UNIT- IV
1. Lipids- classification, structure and functions.
2. Polysaccharides- types, structural features, methods for compositional analysis.
3. Analytical techniques in biochemistry and biophysics for small molecules and macromolecules for quantisation.
4. DNA: Structural features.


UNIT- V
1. RNA: Structure, types and functions.
2. Enzymes: Structure and classification.
3. Vitamins and cofactors: structure, sources and biological properties.
4. High energy phosphate compounds: ATP cycle.

Practicals
1. Titration of amino acids.
2. Colorimetric determination of pK.
3. Model building using space, filling/ ball and stick models.
4. Reactions of amino acids, sugars and lipids, including diagnostic tests.
5. Isolation, purity determination and quantization of cholesterol, DNA and RNA.
6. Quantization of proteins and sugars.
7. Analysis of oils- iodine number, saponification value and acid number.
8. UV, visible, fluorescence and IR spectroscopy and absorption spectra.
9. Separation techniques- centrifugation, chromatography (gel permeation, ion exchange, TLC, etc.), and electrophoresis (PAGE- SDS and AGAROSE).
10. Cleavage of DNA with restriction enzymes and electrophoresis of fragments.
11. Labelling of DNA/RNA plasmid isolation.

Books
1. Biochemical Calculations, Irwinn H. Segel, John Wiley and Sons Inc.
2. General Chemistry, Linus Pauling, W.H. Freeman & Company.
3. Organic Chemistry, DJ Cram and GS Hammond, McGraw Hill.
4. Biochemistry, D. Voet and J. G. Voet, J. Wiley & Sons.
5. Physical Biochemistry, D. Freifilder, W. H. Freeman & Company.
6. Laboratory Techniques in Biochemistry and Molecular Biology, Work and Work.
7. Understanding Chemistry, C. N. R. Rao, Universities Press, Hyderabad, 1999.
8. A Biologist’s Guide to Principles and Techniques of Practical Biochemistry, K. Wilson & K. H. Goulding. ELBS Edition, 1986.
9. Tools of biochemistry by T. G. Cooper.


BIT C 123 Microbial Physiology and Genetics
Theory

UNIT- I
1. The history and development of Microbiology, contribution of Leeuwenhoek, Pasture, Jenner, Coch.
2. Scope of Industrial Microbiology
3.
UNIT-II
1. Microbial physiology, Respiration, Fermentation, Microbial growth: Culture media (Synthetic and complex), batch continious culture, Factors affecting microbial growth.
2.
UNIT- III
1. Metabolic Diversity: Three domain classification of Carl Woese:- Concept of species, Characterization and classification of bacteria, Viruses and fungi, Methods for enumeration of microbial diversity .


UNIT- IV
1. Microbial Diseases: Etiology, prevention and cure, Disease caused by bacteria and viruses, AIDS, tuberculosis, Hepatitis etc.
2. Antimicrobial agents- Antibiotics, Sulfa drugs, Vaccines etc.

UNIT-V
1. Genes, mutation and mutagenesis: UV and chemical mutagens; types of mutations, methods of genetic analysis.
2. Bacterial Genetic System: Transformation, conjugation, transduction, recombination, plasmids and transposing, bacterial genetics map with reference to E. Coli.

Practicals

1. Preparation of liquid and solid media for growth of microorganisms.
2. Isolation and maintenance of organisms by plating, streaking and serial dilution methods, slants and stab cultures, storage of microorganisms.
3. Isolation of pure cultures from soil and water.
4. Growth, growth curve; measurement of bacterial population by turbidometry and serial dilution methods. Effect of temperature, pH, carbon and nitrogen sources on growth.
5. Microscopic examination of bacteria, yeast and molds and study of organisms by gram stain acid fast stain and staining for spores.
6. Study of mutations by ames test.
7. Assay of antibiotics and demonstration of antibiotic resistance.
8. Bacterial transformation.
9. Biochemical characterization of selected microbes.
10. Transduction.
11. One step growth curve of coliphage.

Books
1. General Microbiology, Stainer, R. Y., Ingraham, J. L., Wheelis, M. L., and Painter, P. R. The McMillan Press Ltd.
2. Brock Biology of Microorganisms, Madigan, M. T., Martinko, J. M. and Parker, J. Prentice Hall.
3. Microbiology, Pelczar, M. J., Jr. Chan, F. C. S., and Kreig, N. R., Tata- McGraw- Hill.
4. Microbial Genetics, Maloy, S. R., Cronan, J. E. Jr., and Friefelder, D. Jones and Bartlett Publishers.
5. Microbiology, A Laboratory Manual, Cappuccino, J. G., and Sherman, N., Addison Wesley.
6. Microbiological Applications (A Laboratory Manual in General Microbiology), Benson, H. J., W. C. B., Wm. C. Brown Publishers.


BIT C 124 Computer Applications and Biostatistics
Theory
UNIT- I
1. Brief description and tabulation of data and its graphical representation.
2. Measure of central tendency and dispersion: mean, median, mode, range, standard deviation, and variance. Idea of two types of errors and level of significance, tests of significance (F and t test): chi- square tests.

UNIT- II
1. Simple linear regression and correlation.
2. Introduction of digital computers: organizations; low-level and high- level and languages, the binary number system.

UNIT- III
1. Flow charts and programming techniques.
2. Introduction to programming in Q basic and C.

UNIT- IV
1. Introduction to data structures and database concepts, introduction to internet and its applications.
2. Introduction to MS- office software, covering word processing, spreadsheets and presentation software.

UNIT- V
1. Introduction to Harvard graphics/ Sigma plotter.
2. Computer- oriented statistical techniques: Frequency table of single discrete variable. Bubble sort, computation of mean, variance and standard deviation; t- test, correlation coefficient.
3. Bio- informatics and bio- technology.


Practicals:
1. Presentation of data: Bar diagram, pie diagram, histogram, frequency table.
2. Determination of mean, median & mode.
3. Correlation analysis.
4. Calculation of t,F and Chi square.
5. To write a program in C language to convert Celcius temperature in Fahrenheit
6. To write a program to find greater among two numbers.
7. To write a program to find the area of circle.
Books:
1. Statistical methods- G.W. Snedeear & W.G. Cochran Affiliated East – West press & lowa state University press.
2. Statistics: Theory and practice, M.C. Sharma and G.S. Gulshan. S. Chand & Co. (pvt.) Ltd, New Delhi.
3. Statistical methods in biology, T.N. Bailley, English Language Book society & The English University press ltd.
4. Statistics: Theory and practical problems, D.N. Elhance and P.N. Elhance, Vol. I&II, Kitab Mahal, Allahabad.
5. Computer Fundamentals, P.K. Sinha. BPB publications.
6. Programming in C++ Yashwant Kanitkar, CBS publications, New Delhi.


BIT E 121 BIOINFORMATICS
UNIT-I
1. Introduction: Objectives Multiplicity of data, major bioinformatics databases, data integration, data analysis, Cancer in bioinformatics.
2. Molecular biology and Bioinformatics.
3. Information molecules and in formation flow.
4. Biological database.

UNIT-II
1. Computer architecture.
2. Types of computers.
3. Introduction to internet.
4. Search engines.

UNIT-III
1. Programming languages.
2. Genomics.
3. Proteomics.
4. Phylogenetic analysis.

UNIT-IV
1. Tools for sequence alignment.
2. Alignment of pairs of sequence.
3. Alignment of multiple sequence.
4. Gene Prediction methods.

UNIT-V
1. Visualization and Prediction of Protein structure.
2. Gene mapping, Sequence assembly and gene expression.
3. Microassay technology.
4. Genome sequencing projects.

Practicals:
1. Demonstration of digital computer and its devices.
2. Operating system commands.
3. Document preparation and formatting.
4. Power point presentation.
5. Web development using front page.
6. Website surfing on the basis of Unit V.
7. Sequence analysis.

Books:
1. Bailey, N.T.J.(3rd Ed.) Statistical Methods in Biology.
2. Fundamentals of Information Technology: Alexis Leon, Mathews Leon(1999), Vikas Publishing House and Leon Press Chennai.
3. Fundamentals of Computers: C-Xavier(2003), New Age Publisher, New Delhi.
4. Williams, B.K. and Sawyer, S.C. (2003). The internet & the World Wide Web in Using Information Technology. 5th ed. Tata McGraw-Holl, New Delhi.

Here is the attachment.