#1
6th April 2016, 09:02 AM
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Retention Time in Chromatography
Hi I would like to know the meaning of what retention time means in the chromatography segment as well as what does relative retention time means?
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#2
6th April 2016, 09:04 AM
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Re: Retention Time in Chromatography
Retention time (RT) is a measure of the time taken for a solute to go through a chromatography segment. It is ascertained as the time from infusion to discovery. The RT for a compound is not settled the same number of components can impact it regardless of the possibility that the same GC and segment are utilized. These include: The gas stream rate Temperature contrasts in the broiler and segment Segment corruption Segment length These components can make it hard to think about maintenance times. Regardless of the fact that you utilize the same GC only a couple of days separated, there can be little contrasts in the maintenance time of a compound. Please find the details of the Qualitative analysis relies on comparing the retention times of the peaks in an unknown sample with those of known standards below: Relative Retention Time The utilization of the relative maintenance time (RRT) lessens the impacts of a portion of the variables that can influence the maintenance time. RRT is a statement of a specimen's maintenance time with respect to the standard's maintenance time. |