Pune University MSc medical microbiology entrance exam syllabus

I want to take admission in the MSc medical microbiology in the Pune University? So I am searching for the Pune University MSc medical microbiology entrance exam syllabus? can you please provide me this?

[Arun Vats]

As you say that you want to take admission in the MSc medical microbiology in the Pune University. The Department conducts the written M.Sc. entrance examination in the month of June every year and gives the admission to twenty from the number of applicants as large as 600 to 900.

Nature of M.Sc. Entrance Examination :

Nature of Questions Objective only

Number of Questions 50

Marks per question Two

Total Marks 100

Passing criteria Not Applicable

Negative Marking -1 for each wrong answered question

You are asking for the syllabus for the Pune University MSc medical microbiology entrance exam.

Pune University MSc medical microbiology entrance exam syllabus

F. Y. B. Sc. MICROBIOLOGY
PAPER I – INTRODUCTION TO MICROBIOLOGY
I.* 1. Scope and Applications of Microbiology
2. Basic and applied aspects:
a. Medical Microbiology
b. Immunology
c. Soil and Agricultural Microbiology
d. Food and Dairy Microbiology
e. Geomicrobiology
f. Microbial Genetics and Biotechnology

II. 1. History of Microbiology
a. Discovery of microscope and Microbial world
•Early observations
•Micrographia of Anton von Leeuwenhoek and Robert Hooke
b. Controversy over Abiogenesis
•Aristotle’s notion about spontaneous generation
•Redi’s experiment
•Louis Pasteur’s & Tyndall’s experiment

2. Development of Microbiology in 19th century & Last two Decades
a. Discovery of Microbial role in transformation of organic matter.
•Germ theory of fermentation
•Discovery of anaerobic life & physiological significance of
fermentation
b. Discovery of microbial role in causation of disease
•Surgical antisepsis
•Germ theory of disease – Koch’s postulates & River’s
postulates

3. Developments in 20th century in:
•Vaccination
•Chemotherapy
•Modern Immunology
•Molecular Biology & Biotechnology

III. Diversity of Microbial World
Morphological and other characteristic features of:
Bacteria
Rickettsia
Protozoa
Algae
Fungi
Viruses, viroids and prions
10
Topics I, II and III are for the first term
This topic (I) should be used only for internal evaluation and no questions be
asked in final examination on this topic.

IV. a. Morphological / physiological characters, habitat and significance /
role of the following microbial groups in natural environment /
human health;:
•Gram positive endospore forming rods – Bacillus
•Gram positive nonspore forming rods- Lactobacillus
•Gram positive cocci – Staphylococcus
•Gram negative rods – Salmonella

b. General characters and life cycle of
•Plasmodium spp.
•Animal viruses - Polio virus
•Plant viruses - TMV
•Yeasts – Saccharomyces
•Molds- Penicillium
•Bacteriophage – λ phage

V. Bacterial cytology
1. Comparative account of prokaryotes and eukaryotes 2
2. Studies on chemical composition and structure –function
relationship in bacteria;
Cell wall
Cell membrane
Endospore
Capsule
Flagella
Fimbriae and pili
Ribosomes
Chromosomal & extra-chromosomal material
Cell inclusions ( Gas vesicles, carboxysomes, PHB granules,
metachromatic granules and glycogen bodies)

VI. Molecules that make life
Chemical elements, structure of atoms, molecules and chemical bonds,
chemical reactions, pH and pK, buffers

Chemistry of Biomolecules
Carbohydrates
Lipids
Proteins
Nucleic acids

The topics IV, V & VI are for second term

References:
1. Ingraham J. L. and Ingraham C.A. (2004). Introduction to Microbiology. 3nd
Edition. Thomson Brooks / Cole.
2. Madigan M.T., Martinko J.M. (2006). Brock’s Biology of Microorganisms. 11th
Edition. Pearson Education Inc.
3. Tortora G.J., Funke B.R., Case C.L. (2006). Microbiology: An Introduction. 8th
Edition. Pearson Education Inc
4. Stanier R.Y., Adelberg E.A. and Ingraham J.L. (1987) General Microbiology, 5th
Edition. Macmillan Press Ltd.

PAPER II – BASIC TECHNIQUES IN MICROBIOLOGY
I. Safety in Microbiology laboratory, Possible laboratory hazards, Safety
precautions, Disposal of laboratory waste

II. Microscopy :
Bright field microscopy: Structure, working of and ray diagram; concepts
of magnification, numerical aperture and resolving power
Types, ray diagram and functions of – condenser, eye-pieces and
objectives
Aberration of lenses - spherical, chromatic, comma and astigmatism
Principles, construction, working and applications of:
Dark field microscopy,
Fluorescence microscopy,
Phase Contrast microscopy,
Transmission Electron Microscope and Scanning Electron
Microscope

III. Staining Techniques efinitions: Stain(Basic and Acidic ), Fixative,
Mordant, Decoloriser,Accentuator
Principles of staining techniques for following:: –
•Monochrome staining and Negative staining
•Differential staining - Gram staining and Acid fast staining
•Special staining techniques – Spore and Capsule

IV. Sterilization and Disinfection
1. Physical Agents - Heat, Radiation, Filtration 6
2. Chemical agents and their mode of action - Aldehydes, Halogens,
Quaternary ammonium compounds, Phenol and phenolic
compounds, Heavy metals, Alcohol, Dyes, and Detergents, Ethylene
oxide,
Characteristics of an ideal disinfectant

Topics I, II, III and IV are for first term
V. Cultivation of Microorganisms
1. Introduction to concept of pure culture and methods for pure culture 2
2. Nutritional requirements and nutritional classification 4
3. Design and preparation of media – Ingredients of media and types of
media

4. Techniques of enrichment 2
5. Methods of cultivating protozoa, photosynthetic organisms,
extremophiles, chemolithotrophs

6. Isolation and maintenance of bacterial and fungal cultures 2
7. Culture collections and their role 2
VI. 1. Bacterial Growth
a. Growth Kinetics and growth curve;definitions of Generation time,
Growth rate, specific growth rate

b. Methods of enumeration -
Microscopic methods
Plate counts
Biomass
Chemical methods
Optical density

c. Continuous culture – Chemostat and Turbidostat models 3
d. Diauxic growth 2
e. Synchronous culture 3
Topics V, VI and VII are for second term

References:
1. Ingraham J. L. and Ingraham C.A. (2004). Introduction to Microbiology. 3nd
Edition. Thomson Brooks / Cole.
2. Madigan M.T., Martinko J.M. (2006). Brock’s Biology of Microorganisms. 11th
Edition. Pearson Education Inc.
3. Prescott L.M., Harley J.P., AND Klein D.A. (2005). Microbiology, 6th Edition.
MacGraw Hill Companies Inc.
4. Salle A.J. (1971) Fundamental Principles of Bacteriology. 7th Edition. Tata
MacGraw Publishing Co.
5. Stanier R.Y., Adelberg E.A. and Ingraham J.L. (1987) General Microbiology, 5th
Edition. Macmillan Press Ltd.
6. Tortora G.J., Funke B.R., Case C.L. (2006). Microbiology: An Introduction. 8th
Edition. Pearson Education Inc.
7. Wilson K. and Walker J.M. (2005) Principles and Techniques of Biochemistry
and Molecular Biology. 6th Edition. Cambridge University Press.

PRACTICAL COURSE - BASED ON THEORY PAPER I & II ( 72 )
1. Introduction to laboratory instruments – incubator, oven, autoclave,
colorimeter and pH meter.
2 Structure and working of light and dark field microscope, phase contrast
microscope
3 Observation of microorganisms - Bacteria, Protozoa, Fungi and yeasts, Algae
– from natural habitat
4 Cultivation of microorganisms- Hay infusion broth
5 Enumeration of cells by Neubaeur chamber
6 Preparation of laboratory media (Liquid & solid )
7 Checking sterilization efficiency of – autoclave
8 Aseptic transfer techniques – types – slant to slant, broth to broth, broth to
agar
9 Isolation of bacteria by spread plate, streak plate and pour plate method,
Observation of cultural characters
10 Staining - Monochrome, Negative, Gram
11 Stainings: Cell wall, Capsule, Spore, and metachromatic granules
12 Observation of bacterial motility – Hanging drop, Cragie tube, Swarming
growth
13 Demonstrations: Enrichment of photosynthetic organisms, chemolithotrophs,
anaerobic bacteria, Bacteriophage, Demonstrations: Winogradsky
column,Anaaerobic jar,Enrichment of bacteriophage and plaque formation.
14 Maintenance and revival of cultures on slants and soil
15 Personal Hygiene –
Study of normal flora of skin:
a. Cultivating and observing different morphoforms of bacteria from skin.
b. Effect of soap and disinfectant washing

References:
1. Ingraham J. L. and Ingraham C.A. (2004). Introduction to Microbiology. 3nd
Edition. Thomson Brooks / Cole.
2. Madigan M.T., Martinko J.M. (2006). Brock’s Biology of Microorganisms. 11th
Edition. Pearson Education Inc.
3. Prescott L.M., Harley J.P., AND Klein D.A. (2005). Microbiology, 6th Edition.
MacGraw Hill Companies Inc.
4. Salle A.J. (1971) Fundamental Principles of Bacteriology. 7th Edition. Tata
MacGraw Publishing Co.
5. Stanier R.Y., Adelberg E.A. and Ingraham J.L. (1987) General Microbiology, 5th
Edition. Macmillan Press Ltd.
6. Tortora G.J., Funke B.R., Case C.L. (2006). Microbiology: An Introduction. 8th
Edition. Pearson Education Inc.
7. Wilson K. and Walker J.M. (2005) Principles and Techniques of Biochemistry
and Molecular Biology. 6th Edition. Cambridge University Press.

I am appearing in M.Sc medical microbiology entrance exam of Pune University so I need the syllabus of it can you please provide me?

[Quick Sam]

Pune University conducts the written M.Sc. entrance exam in the month of June every year

Online application for the entrance examination begins in the month of April / May.

Eligibility:
Must have passed B.Sc. Microbiology from recognized University

Syllabus of M. Sc Microbiology Entrance Examination
General Microbiology: Milestones of the historical development of Microbiology- Discovery of
Microorganisms- Contributions of Scientists of Microbiology. Theory of spontaneous generation
and biogenesis. Different types of microscopes, their construction and working principles, Simple
and Compound microscope. Stereomicroscope. Principles, construction and mode of operation of
Scanning and transmission electron microscope, limitations. Preparation of specimens for electron
microscopic studies. Simple staining (positive and negative), differential staining (Gram’s staining
and acid – fast staining), structural staining (Capsule, Flagella, Cell wall and Endospore of
bacteria), nuclear staining, wet mounting method – staining of algae and fungi. Hanging drop
method. Principles and methods of sterilization.
The Microbial World: Groups of microorganisms: Viruses, Prokaryotes, and Eukaryotes. Study of
ultra structure of typical prokaryotic cell and eukaryotic cell, comparative account. General
Principles of classification and nomenclature of microorganisms (Haeckel’s three kingdom
classification and Whittaker’s five kingdom classification). General characteristics of viruses,
classification of viruses, isolation and identification of viruses. Study of structure and replication of
viruses: Bacteriophages T4 phage, Cyanophages. Phytophagenae TMV, Zoophagenae – Influenza
and HIV. Viroids and Prions. Ultra Structure of bacterial cell, classification of bacteria in brief as
per Bergey’s Manual of Systematic Bacteriology. Distribution, structure of typical algal cell.
General thallus structure and reproduction in algae and fungi.
Microbial Growth and Metabolism: Major nutritional types of microorganisms. Nutritional
requirements of microorganisms. Uptake of nutrients – passive and active transport. Microbial
Growth rate and generation time, growth curve – phases of growth and their significance.
Nomenclature, classification, properties of microbial enzymes mode and mechanism of enzyme
action, enzyme regulation, inhibition, cofactors and coenzymes. Photosynthetic microorganisms,
photosynthetic pigments and apparatus in prokaryotes and eukaryotes. Mechanism of
photosynthesis in bacteria. Anaerobic Respiration: breakdown of glucose to pyruvate – EMP, HMP
and ED pathways, Fermentation- conversion of pyruvate to ethanol and lactic acid. Aerobic
Respiration: formation of acetyl CoA from pyruvate, TCA Cycle, Electron transport and oxidative
phosphorylation, efficiency of aerobic and anaerobic respiration as energy yielding processes.
Symbiotic and asymbiotic nitrogen fixation, nodule formation, bacteroids, leghaemoglobin,
mechanism and biochemistry of N2 fixation, amino acid synthesis, proteolysis, nitrification and
denitrification. Biosynthesis of fatty acids, biosynthesis of triglyceriods and phospholipids,
degradation of fatty acids, oxidation of fatty acids.
Microbial Genetics: Chromosomes: Prokaryotic and eukaryotic organization. Recombination in
Bacteria:Transformation, Transduction and Conjugation process – F – factor, Sexduction,
Transposons. Extrachromosomal genetic elements and their importance. Structure and types of
DNA and RNA. Replication – mode and mechanism, replication in prokaryotic DNA – general
methodology, Gene – protein relationship: one gene – one enzyme and one gene – polypeptide
concept. Protien synthesis – transcription and translation, genetic code – features, triplet code,
Wobble hypothesis, nonsense codon, evolution of genetic code, regulation of gene expression in
prokaryotes. Nature and types of mutation. Mutagenic agents: physical and chemical mutagens,
damage and repair of DNA: Photoreactivation & SOS repair
Environmental microbiology: Microbes and atmosphere, Air spora of indoor and outdoor
environment, factors affecting air spora, significance of air-borne microbes, management of airborne
microbes. Microbiology of water: distribution of microorganisms in the aquatic environment.
Water pollution: Sources, water-borne diseases-viral (jaundice), bacterial (cholera) and protozoan,
(amoebic dysentry). Biological indicators of water pollution. Determination of sanitary
quality of water: SPC, Tests for coliform, MPN, IMVIC reactions, membrane filter technique.
Microbiology of waste water: Sources of waste water- domestic, agricultural and industrial,
physical, chemical and microbiological characteristics of waste water.
Waste water treatment: Single dwelling unit – septic tank: municipal waste water treatmentPrimary,
secondary (trickling filter, activated sludge process, oxidation pond), Tertiary (reverse
osmosis, ion exchange method and dialysis) and reclamation of waste water. Solid waste recycling:
Anaerobic digestion process, Biogas and composting
Genetic engineering: Tools involved in genetic engineering: Modifying enzymes: Restriction
enzymes, ligases, methylases. Cloning vehicles – Naturally occurring plasmids, cloning plasmids
(PBR 322 and PUC 18). Viruses as cloning vehicles (DNA, M 13) and hybrid vectors (cosmid,
yac). DNA isolation, gel electrophoresis: Agarose gel-principle and method transformation
methods. DNA libraries: Brief account of Genomic and cDNA libraries – applications. Blottings:
Southern, Northern and Western. DNA sequencing: Brief account of Maxam and Gilbert’s
methods, Sanger’s method, automated sequencing method. Restriction fragment length
polymorphism. Molecular probes (Finger printing). Applications of genetic engineering in
Agriculture, Waste water management, Industries, Human health, Potential problems of genetic
engineering; social impact of genetic engineering.
Microorganisms in agriculture: Biofertilizers: Nitrogen fixing, Phosphate solubilizing and
cellulolytic microbes,mass production of bacterial inoculants (Rhizobium, Azotobacter,
Azospirillum, cyanobacteria) mode of applications, advantages and limitations. Biological control:
mechanism of antagonism, amensalism, competition predation and parasitism. Application of
biological control on field. Biopesticides: types – bacterial, viral, fungal and protozoan, mode of
action, target pests, use of transgenic plants. Rhizosphere microorganisms: Rhizosphere and
rhizoplane, interactions among micro organisms – neutralism, mutualism, commensalisms,
antagonism and parasitism Plant – microbe interaction – mycorrhizae. Plant pathology:
classification of plant diseases, principles of infection and spread of diseases in general.
Food Microbiology: Food as a substrate for growth of microorganism’s .Microbial spoilage of
food and food preservation techniques. Microbial spoilage of food – Fruits, vegetables, meat,
poultry, canned foods. Important methods of food preservations. Physical method- High
temperature, Canning, Low temperature, Drying, radiation and Chemical preservatives. Mycotoxin
with reference to Aflatoxin, Staphylococcal intoxication, Botulism, Salmonellosis. Food safety and
quality control. A brief account of food laws and standards. Sources of milk contamination.
Methods to detect microbial spoilage by SPC, Reductase test, Biochemical changes of milk. Starter
culture and its role. Fermented dairy products. Acidophilus milk, Yogurt, Butter milk, Srikhand.
Types of cheese, Preparation of cheese. Preservation of milk and milk products – Pasteurization,
Sterilization.
Industrial Microbiology: Microorganisms of industrial importance. Types of stock culture. Strain
improvement. Fermentation media : A brief account of Production medium, Inoculum medium,
Raw materials – Molasses and types, corn steep liquor, sulphite waste liquor and whey, Buffers,
Precursors, Inhibitors, and Antifoam agents.
Design of typical fermentors, Devices for aeration, agitation. Fermentation process-Surface,
Submerged and Solid state fermentation: Types-Batch and Continuous fermentation. Down stream
processing – Precipitation, filtration, centrifugation, distillation, cell disruption, solvent recovery,
drying, crystallization Industrial production and its uses of the following: Ethyl alcohol, Wine
Antibiotics – Penicillin. Organic acid – Lactic acid. Enzymes – Amylase, Protease, Single Cell
Protein, Mushroom cultivation, Role of microorganisms for production and recovery of minerals
and petroleum.
Immunology and Medical microbiology: introduction to immune system: Types of immunityInnate
and Adaptive Immunity. Antibody mediated immunity and Cell mediated immunity. Cells
and tissues of immune system – Structure and role of primary lymphoid organs (bone marrow,
thymus), secondary lymphoid organs (spleen, lymph nodes and tonsils) B&T lymphocytes,
phagocytes, killer cells, NK cells.
Antigens – nature and types. Antibodies – Structure of lgG. Classes of antibodies and their
functional diversity, Human blood types and Rh factors, Antigen-antibody reactions-salient
features. Agglutination reaction. Immunotechniques – RIA, ELISA. Complement system.
Immunoprophylaxis – Vaccine-Types – Killed, Live attenuated (bacterial and viral) and Toxoid.
Pathogenesis, clinical symptoms, laboratory diagnosis, epidemiology, prophylaxis and treatment of
the following -Bacterial diseases-Tuberculosis, Cholera, Typhoid, Syphilis. Viral diseases –
Hepatitis, Poliomyelitis, AIDS. Fungal diseases-Candidacies, Dermatomycosis (Tinea – ringworm
infection). Protozoan diseases – Malaria, Trichomoniasis.
Chemotherapy: General characteristics and types of antibiotics.

Contact Details:
Savitribai Phule Pune University
Ganeshkhind, Pune, Maharashtra 411007 ‎
020 2569 6061

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I want syllabuss of msc entance microbiology