PTU and Catechol oxidase

Hello sir I want to know about the Action of Catechol Oxidase on PTU so please provide me information about these two products.

[Kiran Chandar]

The Action of Catechol Oxidase will investigate the inhibition of enzyme activity by specific chemicals called inhibitors. The specific inhibitor used will be phenylthiourea (PTU).

Inhibiting the Action of Catechol Oxidase

Materials

Test tube rack with 3 small test tubes

Calibrated 5-ml pipette

Distilled water

Potato extract

Catechol

Disposable pasteur pipettes with pipette bulb

Phenylthiourea (PTU)

Disposable gloves (optional)

Process

An inhibitor molecule affects an enzyme in one of two ways.

Competitive inhibition takes place when a molecule that is structurally similar to the substrate for a particular reaction competes for a position at the active site on the enzyme.

This ties up the enzyme so that it is not available to the substrate.

Competitive inhibition can be reversed if the concentration of substrate is raised to sufficiently high levels while the concentration of the inhibitor is held constant

Hypothesis

Hypothesize about the nature of inhibition by PTU.

Prediction

Predict the results of the experiment based on your hypothesis