M.Tech in Genetic Engineering course details

Hi can I apply for M.Tech in Genetic Engineering provide me the details regarding this course?

[Quick Sam]

M.Tech. Genetic Engineering or Master of Technology in Genetic Engineering is a postgraduate Genetic Engineering course of duration 2 years.

M.Tech. Genetic Engineering Eligibility

Graduation degree in a field of genetics, biotechnology, microbiology, biochemistry, bioinformatics, botany, zoology or any related fields.

Selection to the postgraduate courses (M.Sc. / M.Tech.) in different universities is through an All India Combined Entrance exam conducted by JNU, New Delhi and to IIT's through GATE in Two year / four semesters M.Tech. courses and through JEE.

The marks of admission and other criteria for this course may vary from University to University.

If you are interested and eligible for this course then you can apply for this course.

M.Tech. Genetic Engineering syllabus-

Subjects of Study-

Molecular Genetics
Biological Chemistry
Immunobiology
Statistical Genetics

Elective I
Recombinant DNA Technology
Genomics and Proteomics
Regulation of Gene Expression
Sequence Analysis & Structure Prediction
Elective II
Elective III
Elective IV
Elective V

Seminar
Project-Phase I
Project-Phase II

Title-

Module I: Human Genetics
Human Physiology
Human Genetics
Pharmacogenomics

Module II: Plant Genetics
Plant Physiology and Development
Classical and Molecular Plant Breeding
Genetic Engineering of Plants

Module III: Medical Genetics
Stem Cell Technology
Cancer Biology
Molecular Mechanisms of Infectious Disease

M.Tech. Genetic Engineering colleges in India

Aryabhatta Knowledge University - Master of Technology in Genetic Engineering
Bharath Institute of Higher Education and Research, Chennai - Master of Technology in Genetic Engineering
Bharath Institute of Science and Technology - Master of Technology in Genetic Engineering
Bharath University, Chennai - Master of Technology in Genetic Engineering
S.R.M Institute of Science and Technology - Master of Technology in Genetic Engineering

I want to take admission in the SRM University M.Tech in Genetic Engineering course? I am searching for the syllabus of the M.Tech in Genetic Engineering course of SRM University? Can you please provide me this?

[Arun Vats]

You are asking for the syllabus of the M.Tech in Genetic Engineering course of SRM University. Here I am uploading a file that contains the SRM University M.Tech in Genetic Engineering course syllabus. You can download this from here.

UNIT I - FUNDAMENTALS OF GENENTICS (9 hours)
Mendelian inheritance: Mendel’s laws - Chromosomes and heredity: linkage
and linkage groups - Gene: gene concept, unit of function, replication,
recombination and repair, transposable elements – complementation analysis
- rII locus, inborn errors of metabolism, one gene/one enzyme hypothesis,
pathways of gene action.

UNIT II - GENOME ORGANIZATION (9 hours)
Genome organization in prokaryotes and eukaryotes - DNA content and Cvalue
paradox - methods to measure DNA content variation - various types of
DNA sequences – simple sequences, repetitive sequences, tandem gene
clusters, satellites, Cot curve, DNA structures: double helix, Z-DNA, BDNA,
mechanism of DNA replication: prokaryotes and eukaryotes.

UNIT III - MECHANISM OF TRANSCRIPTION (9 hours)
Mechanism of transcription: prokaryotes and eukaryotes - Operon and operon
concept - Eukaryotic gene structure and expression - Mechanism of
translation: prokaryotes and eukaryotes, Control of gene expression - RNA
processing and editing, transcriptional, post transcriptional, translational and
post transnational controls

UNIT IV- MOLECULAR GENETICS OF PHAGE AND YEAST
(9 hours)
Phage molecular genetics: genetic organisation - lytic and lysogenic cycle,
regulation of genes - T-odd coliphages – ss DNA phages – RNA phage.
Yeast molecular genetics: genome - mutants and genetic screens – genetic
redundancy – cell type determination – cell cycle regulation of mitotic events
– genetic interaction: two hybrid systems – gal pathway, gene regulation.

UNIT V- MOLECULAR GENETICS OF DROSOPHILA (9 hours)
Drosophila molecular genetics: genome - development genetics – mutants
and genetic screens – P element biology –construction and use of genetic
mosaics. Mouse molecular genetics: directed gene expression – gene dosage
compensation – gene replacement and knockout.

LABORATORY EXPERIMENTS (45 hours)
1. Analysis of genetic markers in bacteria
2. Measurement of growth rate; One step growth curve using a even
phage
3. Induced mutagenesis and isolation of antibiotic resistant and
auxotrophic mutants
4. Enrichment for antibiotic resistant and auxotrophic mutants
5. Genetic mapping by P1 transduction
6. Genetic mapping by conjugation
7. Isolation of specialized transducing phage
8. Transposon mutagenesis

REFERENCES
1. L. Snyder, Molecular Genetics of Bacteria, Blackwell Publishing
Company, Oxford, UK. Third Edition (2007).
2. J.R. Johnston, Molecular Genetics of Yeast - A Practical Approach,
Oxford University Press, UK First Edition (1994).
3. S.B. Primrose and R.M. Twyman, Principles of Genome Analysis and
Genomics, Blackwell Publishing Company, Oxford, UK Third
Edition (2003).
4. L M. Silver Mouse genetics: Concepts and Applications, Oxford
University Press, First Edition (1995).
5. W.H.H. Lodish, A.Berk, and C.A. Kaiser, “Molecular Cell Biology”,
Freeman & Co Ltd. Third edition (1995).
6. J.D. Watson, T.A. Baker, S. P. Bell, A. Gann, M. Levine, R. Losick,
“Molecular Biology of the Gene”, Pearson Education Inc, Fifth
Edition (2004).
MANUALS
1. A. Sambrook, and D.W. Russel, Molecular Cloning. A Laboratory
Manual. Volume 1-3. Cold Spring Harbor Laboratory Press, New
York, USA, Third Edition (2001).

Course Code Course Title L T P C
GN2002 BIOLOGICAL CHEMISTRY 3 0 3 4
Total Contact Hours – 90
PURPOSE
To learn the chemical basis of biological system.

INSTRUCTIONAL OBJECTIVES
1. To understand the chemistry and metabolic disorders of
carbohydrates and lipids
2. To understand the chemistry and metabolic disorders of proteins and
nucleic acids
3. To give an knowledge on hormones and their functions and disorders
4. To understand the principle applications analytical techniques.

UNIT I - CARBOHYDRATES AND LIPIDS (10 hours)
Carbohydrates and lipids – Classification and metabolism - metabolic
disorders - Biochemistry of Diabetes mellitus, Glycogen storage diseases,
disorders of lipid metabolism

UNIT II - PROTEIN AND NUCLEIC ACIDS (9 hours)
Protein and nucleic acids – Biochemistry, functions and metabolic disorders,
PEM, biogenic amines, neurotransmitters, nucleic acid metabolism -
inhibitors and disorders.

UNIT III - STEROID HORMONES (8 hours)
Hormone - General mechanism of action of hormones, chemistry, synthesis
of steroid hormones, functions. Disorders –hyper and hypo conditions.

UNIT IV - PEPTIDE HORMONES AND AMINOACID
DERIVATIVES (8 hours)
Cell signaling - Hormone receptors, polypeptide hormones, & thyroid
hormones. Chemistry & functions. Hormones of pancreas, and parathyroid.
Local hormones. Clinical disorders of hormones.

UNIT V -ANALYTICAL TECHNIQUES (10 hours)
Principles, instrumentations and applications of NMR, HPLC, FPLC, GC,
GC-MS, LC-MS, MALDI-ToF.

LABORATORY EXPERIMENTS (45 hours)
1. Preparations of solutions buffers and measurement of pH
2. Analysis of isoenzymes
3. Analysis of blood glucose level
4. Analysis of blood total proteins
5. Protein purification using FPLC
6. HPLC
7. GC

REFERENCES
1. MN Chatterjea, Shinde Rana, “Textbook of Medical biochemistry”
jaypee brothers medical publications 8 the edition, 2011.
2. D. Voet and J.G. Voet, “Fundamentals of Biochemistry”. Wiley
Publishers Second Edition (2005)
3. A.L. Lehninger, D.L. Nelson, and M.M. Cox, “Principles of
Biochemistry”, W. H. Freeman Publications Fifth Edition (2008)
4. Laboratory Manual

Course Code Course Title L T P C
GN2003 IMMUNOBIOLOGY 3 0 3 4
Total Contact Hours - 90
PURPOSE
To introduce the science of immunology and detailed study of various types
of immune systems and methods used in immunology.

INSTRUCTIONAL OBJECTIVES
1. To understand the basic concepts of immunology
2. To know about transplantation immunology and auto immunity
3. To learn the methods used in immunology (immunotechniques)
THEORY

UNIT I - CONCEPTS OF IMMUNOLOGY (9 hours)
General principles of immune system, molecules, cells and tissues of immune
system, primary and secondary lymphoid organs (thymus, bursa of fabricius,
lymph nodes, spleen), B and T lymphocyte and their functions, lymphocyte
cell mediated cytotoxicity.

UNIT II - ANTIGENS AND ANTIBODIES (9 hours)
Concepts of antigen, antigenic determinant, antigenicity, immunogen and
immunogenicity, factors affecting antigenicity, hapten, carrier effect, cross
reactivity, adjuvtants, Freund’s adjuvants and its significance,
immunoglobulin, structure of immunoglobulin, types and properties of
immunoglobulin, theories of antibody formation, clonal selection, Ig genes,
immunoglobulin synthesis and metabolism, and antibody diversity.

UNIT III - HUMORAL AND CELL MEDIATED IMMUNITY(9 hours)
MHC, MHC antigen- Class I, Class II, Class III, antigen presentation, MHC
restriction, immune response gene, immune response, humoral and cell
mediated immune response, BCR, TCR & generation of biodiversity,
lymphocytes, T cells regulation, graft rejection.

UNIT IV - ANTIGEN- ANTIBODY REACTION (9 hours)
Physico-chemical basis of Ag-Ab interaction, avidity, strength of binding
between Ag and Ab and its measurement, detection of Ag-Ab interaction,
precipitation, agglutination and complement fixation, complement system,
and cytokines.

UNIT V - IMMUNOTECHNIQUES (9 hours)
One and two dimensional, single radial immuno diffusion, Ouchterlony
immno diffusion, rocket immunoelectrophoresis, CIE, Graber and William
technique, direct and indirect agglutination, ELISA, Direct, indirect and
Sandwich immunofluorescence, hybridoma technology and monoclonal
antibodies, Abezyme technique, Antiserum production, immuno
histocompatibility, location of cells in tissues, immunoblotting, flow
cytometry.

LABORATORY EXPERIMENTS (45 hours)
1. Antibody production
2. ELISA
3. Western blotting
4. Flowcytometry.
5. Single Radial Immuno Diffusion
6. Double diffusion.
7. Rocket Immunoelectrophoresis.
8. Counter- Current Immunoelectrophoresis.
9. Characterization of Immunoglobulins by SDS-PAGE

REFERENCES
1. P. Delves, S. Martin, D. Burton and I. Roitt, Essential Immunology,
Wiley-Blackwell Publishers, UK , Eleventh Edition (2006).
2. J. Kuby, Immunology, W H Freeman & Co Publishers, Third Edition
(1997).
3. F.C. Hay and O.M.R. Westwood, Practical immunology, Wiley-
Blackwell Publishers Fourth Edition (2002).
4. Immunology - A Short Course, Third Edition E. Benjamin, G.
Sunshine, and S. Leskowitz, “Wiley-Liss Publishers”, New York.
(1996).
5. Fourth Edition D.P. Stites, J.D. “Basic and Clinical Immunology”,
Stobo and J.V. Wells, Appleton & Lange Publishers. (1982)

MANUALS
1. Laboratory Manual

Total Contact Hours - 60
PURPOSE
The purpose of the course is to impart knowledge on different advanced biostatistical
methods for its applications in bio-engineering related disciplines.
The emphasis will be more on solving practical problems with basic
understanding of the theoretical concepts.

INSTRUCTIONAL OBJECTIVES
At the end of the course, the students should able to understand
1. Analysis of biological data to draw valid inferences
2. To understand the working of statistical methods and its applications
to several situations
3. To develop statistical models and to study the several characteristics
of biological data structures

UNIT I - BASIC STATISTICAL MEASURES, CORRELATION AND
REGRESSION (12 hours)
Basic ideas of Measures of central tendency, Measures of dispersion,
Skewness and Kurtosis. Correlation and Regression theory, Testing the
significance of an observed correlation coefficient, observed regression
coefficient and observed partial regression coefficient, Testing difference
between to partial regression coefficient, Multiple regression model.

TUTORIALS
(i) Computation of Mean, Median, Mode, Standard Deviation, Coefficient
of Variation, Coefficient of Skewness.
(ii) Test for Correlation coefficient and Regression coefficient
(iii) Test for partial regression coefficient and for the difference between
two partial regression coefficient

UNIT II - CONTINGENCY TABLES (12 hours)
Chi-square Test-2x2 tables, rxc tables, McNemar’s test, The odd ratio,
Berkson’s fallacy.
Multiple 2x2 contingency tables: Simpson’s Paradox, The Mantel-Haenszel
method, Test of homogeneity, Test of association.

TUTORIALS
(i) McNemar’s test to evaluate hypothesis about the data.
(ii) Computation of The Odd Ratio and Simson’s Paradox.
(iii) Mantel-Haenszel method to combine the information in a 2x2 table.

UNIT III - TWO SAMPLE HYPOTHESIS (12 hours)
Testing for difference between two means, Testing for difference between
two variances, Two sample Rank Testing – The Mann – Whitney Test,
Testing for difference between two Median.

TUTORIALS
(i) One tailed hypothesis and two tailed hypothesis for a two sample Ttest
(ii) An one tailed variance ratio test and two tailed variance ratio test
(iii) One tailed Mann-Whitney Test

UNIT IV- MULTI-SAMPLE HYPOTHESIS (12 hours)
A single factor Analysis of Variance, The Kruskal–Wallis single-factor
analysis of variance by ranks. Bartlett’s test for homogeneity of
variances,Testing for homogeneity of coefficient of variation.

TUTORIALS
(i) Kruskal-Wallis test
(ii) Bartlett’s test
(iii) Test for homogeneity of coefficient of variation

UNIT V- MULTIPLE COMPARISONS (12 hours)
The Tukey test – The Newman-Keuls test – Dunnett’s test - Scheffe’s
Multiple Contrast.

TUTORIALS
(i) The Tukey test with unequal sample sizes
(ii) The Newman-Keuls multiple range test
(iii) Dunnett’s test for comparing a control mean to each other group mean
(iv) Scheffe’s test for multiple contrast

REFERENCES
1. Jerold H.Zar, “Bio-statistical Analysis, Pearson Education Inc.,
Dorling”Kindersley (India) Pvt. Ltd., New Delhi, 4th Edition 2009.
2. Marcello Pagano and Kimberlee Gaurveau, Principles of “Bio-
Statistics”, Duxbury, Thomson Asia Pvt. Ltd., Singapore, 2004.
3. Ronald E. Walpole, Raymond H. Myers, Sharon L. Myers and Keying
Ye: “Probability & Statistical for Engineers and Scientists”, Pearson
Education, Inc., Dorling Kindersley (India) Pvt. Ltd., New Delhi,
2007
.
SEMESTER II

Total Contact Hours - 90
PURPOSE
To learn the molecular techniques that is required to be a successful genetic
engineer of plants, animals and microorganisms.

INSTRUCTIONAL OBJECTIVES
1. To learn the gene cloning methods in theory and practice
2. To learn genetic engineering of living organism for human benefit

THEORY
UNIT I - ENZYMES AND VECTORS IN GENE CLONING (9 hours)
Restriction enzymes, methylases, DNA polymerases, reverse transcriptase,
terminal transferase, alkaline phosphatase, polynucleotide kinase, ligase,
DNase and RNase. Structural and functional organization of plasmids,
plasmid replication, stringent and relaxed plasmids, incompatibility of
plasmid maintenance. Lambda phage vectors.

UNIT II- POLYMERASE CHAIN REACTION (12 hours)
PCR, gene isolation by PCR, primer design – gene specific primers, nested
primers, degenerate primers, optimization of PCR components and thermal
conditions, PCR set up with proper controls, types of PCR – inverse PCR,
nested PCR, TAIL PCR, LAMP, cloning of PCR products – TA cloning,
blunt end cloning, TOPO cloning, cloning with added restriction sites,
GATEWAY cloning, semi quantitative RT-PCR, real-time PCR with SYBR
and Taqman probe, site directed mutagenesis (PCR and non-PCR methods).

UNIT II - GENE CLONING METHODS (8 hours)
Cohesive end cloning, blunt end cloning, cloning using adapters, linkers and
homopolymer tailing, GATYWAY cloning, ligation free cloning,
Construction of cDNA library, subtractive cDNA library, normalized cDNA
library, genomic DNA library.

UNIT IV- GENE & PROMOTER ISOLATION (8 hours)
Methods of screening the libraries using nucleic acid and antibody probes,
functional screening, screening by complementation, cloning of genes by
PCR, RT-PCR, RACE-PCR, artificial gene synthesis, constitutive and
inducible promoters, tissue specific promoters, promoter identification from
gene expression data, promoter deletion studies, reporter genes for promoter
deletion studies.

UNIT VI - GENETIC ENGINEERING OF LIVING ORGANISMS
(8 hours)
Expression and purification of recombinant proteins in E.coli, yeast,
Baculovirus, animal
cell lines, transgenic plants and transgenic animals.

LABORATORY EXPERIMENTS (45 hours)
1. Designing cloning strategies
2. Cloning using restriction enzymes
3. Cloning of PCR products
4. Cloning in expression vector
5. Induction of expression of recombinant protein
6. Purification of recombinant proteins using His Tag
7. Automated DNA sequencing

REFERENCES
1. S.B. Primrose, S.B. and R.M. Twyman, “Principles of Gene
Manipulation and Genomics”, Blackwell Publishing Company,
Oxford, UK Third Edition (2006).
2. T.A. Brown, Gene Cloning and “DNA Analysis: An Introduction”,
Wiley-Blackwell, UK. Fifth Edition (2006).
3. M. Innis, T. White and J.J. Sninsky, PCR Protocols: A Guide to
“Methods and Applications”, Academic Press First Edition (1990).
4. S. Ying, Generation of cDNA Libraries: “Methods and Protocols”,
Humana Press First Edition (2003)