JNU MSc Biotechnology previous year question paper

Kindly provide me the JNU MSc Biotechnology previous year question paper…………..

[Kiran Chandar]

Here is the JNU MSc Biotechnology previous year question paper:

1. Endodermis in plants is modified parenchyma tissue. Which of the following
combinations of molecules depicts composition of its cell wall material?
(a) Cellulose, pectin, hemicellulose
(b) Cellulose, pectin, hemicellulose, cutin
(c) Cellulose, pectin, hemicellulose, lignin
(d) Cellulose, pectin, hemicellulose, suberin
2. Endosperm contains
(a) one nucleus per cell
(b) two nuclei per cell
(c) three nuclei per cell
(d) four nuclei per cell
3. Which one of the following is a synthetic auxin?
(a) IAA
(b) GA
(c) IBA
(d) NAA
4. One of the important consequences of geographical isolation is
(a) preventing speciation
(b) speciation through reproductive isolation
(c) random creation of new species
(d) no change in the isolated fauna
5. Bacterial growth in the logarithmic/ exponential phase is expressed as a power of
2, i.e., 2n. If the value of n is 11, then what would be the number of cells in the culture?
(a) 1100
(b) 1024
(c) 512
(d) 2048

6. Protein synthesis will be inhibited by chloramphenicol in
(a) chloroplasts, mitochondria, E. coli
(b) chloroplasts, mitochondria, yeast cells
(c) chloroplasts, mitochondria, amoebae
(d) chloroplasts, mitochondria, lymphocytes
7. Which urinary pathogen 'swarms' across agar surfaces and may cause bladder and
renal calculi (stones)?
(a) Citrobacter freundii
(b) Enterobacter aerogenes
(c) Proteus mirabilis
(d) Klebsiella oxytoca
8. Which of the following is not true about bacterial sporulation?
(a) Sporulation occurs in response to nutritional deprivation or stress
(b) It is representative of bacterial cell differentiation
(c) Sporulation leads to increase in bacterial numbers
(d) Spores are tolerant to desiccation, heat and ultraviolet radiations
9. Hydroxyproline and hydroxylysine are rare amino acids. These are found in
(a) haemoglobin
(b) casein
(c) collagen
(d) keratin
10. The final electron acceptor in non -cyclic photophosphorylation is
(a) NADP+
(b) ATP
(c) 02
(d) cytochrome c
11. Which of the following properties of water make it an ideal solvent in biological systems?
(a) High heat capacity, high heat of vaporization, high heat of fusion, high surface
tension
(b) Low heat capacity, high heat of vaporization, low heat of fusion, high surface
tension
(c) High heat capacity, low heat of vaporization, high heat of fusion, low surface
tension
(d) Low heat capacity, low heat of vaporization, low heat of fusion, low surface tension

12. Entropy follows the order
(a) gas > liquid > solid
(b) solid > liquid > gas
(c) solid > gas > liquid
(d) solid = gas = liquid
13. - is the structural and functional unit of the nervous system.
(a) Glial cell
(b) Axon
(c) Neuron
(d) Synapse
14. Jejunum is
(a) the upper part of the small intestine
(b) the central part of the small intestine
(c) the lower part of the small intestine
(d) the upper part of the large intestine
15. A shark's blood is isotonic to the surrounding seawater due to reabsorption of which of
the following compounds?
(a) Ammonia
(b) Uric acid
(c) NaCI
(d) Urea
16. The correct sequence of spermatogenetic stages in a mature human testis is
(a) spermatogonia-spermatocyte-spermatid-sperms
(b) spermatid-spermatocyte-spermatogonia-sperms
(c) spermatogonia-spermatid-spermatocyte-sperms
(d) spermatocyte-spermatogonia-spermatid-sperms

17. "All biological catalysts are proteins." The statement is no more valid because of the
discovery of
(a) ribonucleases
(b) ribozymes
(c) abzymes
(d) zymases
18. The necessary ingredients for DNA synthesis are mixed together in a test tube. The DNA
polymerase is from Thermus aquaticus and the template is from a human cell. The DNA
synthesized would be
(a) most similar to human DNA
(b) most similar to T. aquaticus DNA
(c) a mixture of T. aquaticus and human DNA
(d) most similar to human RNA
19. A group of genes which are transcribed together as a unit is called
(a) promoter
(b) operon
(c) operator
(d) repressor
20. Which of the following statements is not correct with respect to DNA replication?
(a) RNA primer is required for both leading and lagging strand syntheses
(b) The synthesis of lagging strand begins after the synthesis of the leading strand
(c) Okazaki fragments are formed both in eukaryotic as well as prokaryotic DNA
replication
(d) The 3' -OH end of either DNA or RNA can function as primer for DNA polymerase
/66-A 6
21. The entropy during formation of hydrogen bonding in ortho-hydroxybenzoic acid and in
para-hydroxybenzoic acid will, respectively
(a) increase and decrease
(b) decrease and decrease
(c) increase and increase
(d) decrease and increase
22. Which of the following molecules has a dipole moment?
(a) CS2
(b) CO2
(c) CHC13
(d) CH2C12
23. SN 1 reaction is favored by
(a) non-polar solvents
(b) more number of alkyl groups on the carbon atom attached to the halogen atom
(c) small groups on the carbon atom attached to the halogen atom
(d) more number of phenyl groups on the carbon atom attached to the halogen atom
24. Which of the following molecules has the lowest bond length between two carbons?
(a) Ethane
(b) Cyclohexane
(c) Benzene
(d) Ethylene
25. Which energy state of molecules is affected by microwave radiation?
(a) Nuclear
(b) Vibrational
(c) Electronic
(d) Rotational

26. Which one of the following will undergo bromoform reaction?
(a) Benzaldehyde
(b) Crotonaldehyde
(c) Ethanol
(d) Tertiary butanol
27. Which one of the following aromatic electrophilic substitutions is fully reversible?
(a) Nitration
(b) Friedel-Crafts alkylation
(c) Sulfonation
(d) Halogenation
28. Nitration of bromobenzene leads to the formation of
(a) ortho-bromonitrobenzene and para-bromonitrobenzene
(b) meta-bromonitrobenzene only
(c) meta-bromonitrobenzene and para-bromonitrobenzene
(d) Both (a) and (b)
29. Which one of the following compounds shows trigonal bipyramidal structure?
(a) CH4
(b) PCI 5
(c) NH3
(d) F20
30. Which one of the following is the correct order of acidity?
(a) HNO3 > HCl > H2S04 > CH3COOH
(b) CH30OOH > H2SO4 > HNO3 > HCl
(c) H2SO4 > HNO3 > HCl > CH3COOH
(d) H2SO4 > CH3COOH > HNO3 > HCl

31. The Edman reagent reacts on
(a) the N-terminal amino acid of a peptide
(b) the C-terminal amino acid of a peptide
(c) both the terminal amino acids of a peptide
(d) aromatic amino acids, selectively
32. An example of a colloidal gel wherein liquid is dispersed in solid is
(a) milk
(b) cheese
(c) mist
(d) paint
33. Which compound upon ozonolysis will give a diketone?
(a) 1,3-Butadiene
(b) 2,3-Dimethyl but-2-ene
(c) 2-Methyl but-2-ene
(d) But-2-ene
34. The compound having the same number for oxidation state and covalency is
(a) PtCI2 •(H20)2
(b) [AICI(H20)5]2+
(c) K3[Fe(CN)6]
(d) CuSO4•(H20)5
35. Which one of the following solutions would show the highest depression of freezing
point?
(a) 0.1 M LiCI in water
(b) 0.1 M LaCI 3 in water
(c) 0.1 M MgCl 2 in water
(d) 0.1 M NaBr in water

36. Cells divide every 2 hours in a reactor. If at time It = 0, the cell count is 105 cells/mL,
then the time required for the cell count to reach 108 cells/mL is approximately
(a) 10 hours
(b) 15 hours
(c) 20 hours
(d) 25 hours
37. If sin x = 2 and it < x < n, then the value of cos x is
(a)
2
(b)
2
(c)
4
1
(d)
2
4
38. In the expansion of (x- 11 , the constant term is
x/
39. Jex[f(x)+ f'(x)Idx is equal to
(a) exf(x)+C
(b) ex{f(x)+f'(x)}+C
(c) ex(f(x)+C)
(d) e x f (x) + f (x) + C
40. The number of solutions of x+2y= $ 2x+4y= 10 is
(a) zero
(b) unique
(c) finite but not unique
(d) infinite

41. Let f : {1, 2, $ 4, 5} -4 {a, b, q d} be a function. Which of the following statements is
true?
(a) f can be one-one function
(b) f is onto function
(c) f cannot be one-one function
(d) f cannot be onto function
42. The period of the function cosx+cosl Xl+cosi
x)
is
2 3
(a) 2n
(b) 4n
(c) 67E
(d) 12n
43. Consider two unit circles (with radius one) whose centres are at (0, 0), (0, 2). The
number of common tangents to these circles is
(a) 0
(b) 1
(c) 2
(d) 3
44. If sinx+siny+sinz= 3 for some x, 4 z, then cosx+cosy+cosz is equal to
(a) 0
(b) I
(c) 2
(d) 3
45. The function f(x) =(x( is
(a) continuous but not differentiable in R
(b) continuous and differentiable in ]R
(c) not continuous but differentiable in 1R
(d) neither continuous nor differentiable in Ilt
46. The pressure of the gas in a constant volume gas thermometer is 80 cm of mercury in
melting ice at 1 atm. When the bulb is placed in a liquid, the pressure becomes 160 cm
of mercury. The temperature of the liquid is
(a) 54630 K
(b) 53620 K
(c) 52610 K
(d) 516.05 K

47. Two point charges +10 pC and +20 pC are placed at a separation of 2 cm. The electric
potential due to the pair at the middle point of the line joining the two charges is
(take, 1/ (4,[e0) = 9 x 109 N m 2 C-2 )
(a) 9 MV
(b) 13.5 MV
(c) 27 MV
(d) 54 MV
48. Two capacitors of capacitance 10 pF and 20 pF are joined in series and connected to a
battery 30 V. Then the charge on each capacitor is
(a) 50 pC
(b) 75 RC
(c) 100 pC
(d) 200 pC
49. The centre of a wheel rolling on a plane surface moves with a speed uo . A particle on the
rim of the wheel at the same level as the centre will be moving at speed of
(a) zero
(b) u0
(c) uo'
(d) 2 uo
50. Two satellites A and B move round the earth in the same orbit. If the mass of B is twice
the mass of A, then
(a) the speeds of A and B are equal
(b) the potential energy of earth + A is same as that of earth + B
(c) the kinetic energies of A and B are equal
(d) the total energy of earth + A is same as that of earth + B
51. A ball of 1 kg dropped from a height of 3 m rebounds only 2.5 m after hitting the
ground. The amount of energy converted to heat is
(a) about 1 J
(b) about 1.5 J
(c) about 2 J
(d) more than 2 J

52. Two simple pendulums are of length 40 cm and 160 cm respectively . Then what is the
ratio of their time periods?
(a) 1 : 1
(b) 1 : 2
(c) 1 : 3
(d) 1 : 4
53. Three unequal charges Q1, Q2 and Q3 are kept as shown in the figure. The magnitude of
the electric field due to charges Qi and Q2 at Q3 is
1C
Qi'- 4 cm
2C
•Q2
54. The energy of the first excited state of quantum harmonic oscillator is
(a) h/2n
(b) h/ 67c
(c) 3h/4a
(d) 5h/4n
55. A projectile is launched at an angle of 300 from the horizontal with an initial velocity
of v. The ratio of the initial velocity to the velocity of the projectile when it reaches its
maximum height is
(a) 1 : 1
(b) 2 : 1
(c) 3 : 1

56. 1374 identical balls are taken each weighing 252 g and the volume of water displaced
by them is 33.3 litres. A proper estimate of the density is
(a) 1040 kg/m 3
(b) 1039.7 kg/m3
(c) 1039.78 kg/m3
(d) 1039784 kg/m3
57. An object of mass 20 g is thrown vertically upward at 10 m/s. The work done by the
force of gravity during the time the object is going up (till the time it reaches a maximum
height) is
(a) zero
(b) 1 J
(c) -1 J
(d) not possible to calculate
58. A battery of 2 V and internal resistance 0.5 i2 is connected across a resistance of 9.5 a
How many electrons cross through a cross-section of the resistance in 1 second?
(Charge of one electron is 1.6 x 10-19 C)
(a) 125x1019C
(b) 1.25x1019C
(c) 125 x 1018 C
(d) 125 x 1018 C
59. The current supplied by the battery in the circuit as shown in the figure is
124
3V
(a) 3 A
(b) 2 A
(c) 1 A
(d) 0 A
60. If the potential across a 100 4 resistor is 5 V, then the current flowing through the
resistor is
(a) 020 A
(b) 0.10 A
(c) 0.05 A
(d) 0025 A
/66-A 14

Answer any sixty questions
61. The characteristics of Phaeophyta (brown algae) are
(a) fucoxanthin, filamentous thalloid plant body, laminarin
(b) chlorophyll b, unicellular, starch
(c) fucoxanthin, unicellular, starch
(d) chlorophyll b, thalloid filamentous, laminarin
62. Some anatomical and morphological features of angiosperms are listed below :
(i) Reticulate venation, (ii) Parallel venation, (iii) Rings of vascular bundles, (iv) Scattered
vascular bundles, (v) Vascular bundle with cambium, (vi) Vascular bundle without
cambium, (vii) Flower with distinct petals and sepals, (viii) Flower with no distinct petals
and sepals
Which one of the following combinations of features would monocotyledons have?
(a) i, iii, vi, viii
(b) ii, iv, vi, viii
(c) -i, iii, v, vii
(d) ii, iii, vi, vii
63. 80 g of a forest soil sample was heated at 110 °C to constant dry soil mass of 60 g. It
was then heated repeatedly to red-hot in a crucible. The mass of the soil was now
found to be 30 g. What is the content of water and organic matter of forest soil?
(a) Water 20 g, organic matter 50 g
(b) Water 50 g, organic matter 20 g
(c) Water 60 g, organic matter 30 g
(d) Water 20 g, organic matter 30 g
64. The alkaloid vinblastine which is used to treat leukemia is extracted from
(a) Atropa belladonna
(b) Rauwolfia serpentina
(c) Papaver somnfferum
(d) Catharanthus roseus
65. Infectious RNA and infectious proteins are, respectively, referred to as
(a) viroids and prions
(b) viroids and viruses
(c) prions and pathogens
(d) viruses and pathogens

66. The characteristic features of pteridophytes are
(a) haploid sporophyte, vascular tissues, diploid spores
(b) haploid sporophyte, vascular tissues, haploid spores
(c) diploid sporophyte, vascular tissues, haploid spores
(d) diploid sporophyte, vascular tissues, diploid spores
67. Periderm serves as secondary protective tissue against desiccation and mechanical
injuries in woody plants and it is formed by the activity of
(a) phellem
(b) pericycle
(c) phelloderm
(d) phellogen
68. The most common type of embryo sac (Pogonatum type) in angiosperms consists of
(a) one egg cell, two synergid cells, two antipodal cells and two central cells
(b) one egg cell, two synergid cells, three antipodal cells and one central cell
(c) two egg cells, one synergid cell, three antipodal cells and two central cells
(d) two egg cells, two synergid cells, two antipodal cells and one central cell
69. The photon absorption and subsequent transfer of energy towards the reaction centre
during photosynthesis can be given as
(a) chlorophyll a -> chlorophyll b -> carotenoids -> P680
(b) P680 -> carotenoids -, chlorophyll a -* chlorophyll b
(c) carotenoids -> chlorophyll b -> chlorophyll a -> P680
(d) chlorophyll b -> chlorophyll a -> carotenoids -* P680
70. The volume of a bacterial cell is 10-2 cubic micron. How many cells would be present in
a cylinder of 5 mL?
(a) 5x 108
(b) 5x101°
(c) 5x 1012
(d) 5 x 1014
71. Stability of low copy number plasmid in bacterial cells is ensured by
(a) maintenance of precise copy number before cell division
(b) cell division occurring only after replication of plasmid is completed
(c) synchronous replication of plasmid and chromosome
(d) coordination of replication and partitioning of plasmid

72. Some bacterial pathogens have become resistant to penicillin because
(a) they have acquired effective drug efflux systems
(b) the drug is sequestered by penicillin-binding protein
(c) alternate penicillin-binding proteins that do not bind to penicillin come into play
(d) selective mutation increases the concentration of intracellular penicillin -binding
protein
73. Incompatibility among plasmids within the bacterial cell happens
(a) when two plasmids with different on hamper replication of one another
(b) when two plasmids compete for the same diffusible molecule for replication
(c) when two plasmids share the same diffusible molecule for copy number control
(d) when two plasmids have different cis elements that are essential for replication
74. The basal body of flagella has a small central rod inserted into a system of rings and in
Gram-positive bacteria this consists of
(a) L, P, S and M rings
(b) P, S and M rings
(c) L and P rings
(d) S and M rings
75. The carboxysomes of photosynthetic and chemoautotrophic bacteria contain mainly
(a) malate dehydrogenase
(b) cellulase
(c) ribulose bisphosphate carboxylase
(d) alkaline phosphatase
76. Gluconobacter and Acetobacter are used in industry for the production of ethanol.
However, Gluconobacter is popularly known as `underoxidizer' as it
(a) oxidizes only a very small amount of ethanol to acetic acid
(b) does not oxidize acetic acid further to carbon dioxide
(c) converts some amount of acetic acid to ethanol
(d) also oxidizes acetic acid further to carbon dioxide

77. Platyhelminthes have characteristic features such as
(a) triploblastic, radial symmetry, segmented, acoelomate
(b) diploblastic, bilateral symmetry, unsegmented, coelomate
(c) triploblastic, coelomate, radial symmetry, unsegmented
(d) triploblastic, acoelomate, bilateral symmetry, unsegmented
78. A poikilothermic organism living in the Arctic would have, compared to one living in the
temperate climate zone, a plasma membrane richer in
(a) cholesterol
(b) long-chain fatty acids
(c) protein
(d) unsaturated fatty acids
79. Which of the following types of lipid is present abundantly in the membrane of a cell?
(a) Sulpholipids
(b) Glycerophospholipids
(c) Galactolipids.
(d) Sphingolipids
80. Which of the following is diploid?
(a) Primary polar body
(b) Secondary polar body
(c) Spermatid
(d) Primary spermatocyte

81. Contractile vacuoles in some protists pump water out of the cell . as the surrounding
environment is
(a) hypertonic
(b) hypotonic
(c) isotonic
(d) toxic
82. Which one of the following is not a characteristic of cancer cells?
(a) Reduced cell adhesion
(b) Disorganization of cytoskeleton
(c) Presence of predominant GM3 ganglioside
(d) Decrease in negative surface charge of cell membranes
83. Proteins synthesized by the rough ER are meant
(a) for transporting to various organelles
(b) to build more membranes in the cell
(c) to digest food in lysosomes
(d) to be exported from the cell
84. In testes, high concentration of testosterone is maintained through its secretion by
(a) Mast cells
(b) Sertoli cells
(c) Leydig's cells
(d) Epithelial cells

85. A distinct class of cysteine proteases that mediates apoptosis is that of
(a) cysteinases
(b) caspases
(c) sulphydryl hydrolases
(d) lysosomal hydrolases
86. Purity of an enzyme at various stages of purification can be best measured by
(a) total enzyme activity
(b) percent recovery of the enzyme
(c) total protein
(d) specific activity of the enzyme
87. The correct order for the electronegativity of the elements listed below is
(a) S>N>O>C>H
(b) O>N>S>C>H
(c) N> O>S>C>H
(d) C>O>H>N>S
88. In mitochondria , protons would accumulate in the
(a) outer membrane
(b) inner membrane
(c) intermembrane space
(d) matrix
89. The total energy released by the conversion of glucose to ethanol during alcoholic
fermentation in yeast is 210 kJ per mole. The alcoholic fermentation yields 2 ATP. The
energy contained in 2 ATP molecules is 61 .2 W. The efficiency of transfer of energy
during this fermentation is approximately
(a) 39%
(b) 49%
(c) 29%
(d) 59%

90. In a bilayer cell membrane, lipid molecules are packed in such a way that
(a) the hydrophilic tails are sandwiched between all the hydrophobic heads
(b) the hydrophilic heads are sandwiched between all the hydrophilic tails
(c) the hydrophobic tails are sandwiched between hydrophilic, heads
(d) the hydrophobic heads are sandwiched between hydrophilic' tails
91. In the third stage of aerobic respiration, the final acceptor of electrons from glucose is
(a) water
(b) hydrogen
(c) oxygen
(d) cytochrome aa3
92. Which among the following arrangements of the protein estimation methods is from the
least to the most sensitive technique?
(a) Biuret method; UV-spectrophotometric method; Lowry's method; Bradford method
(b) UV-spectrophotometric method; Biuret method; Lowry's method; Bradford method
(c) Biuret method; UV-spectrophotometric method; Bradford method; Lowry's method
(d) UV-spectrophotometric method; Biuret method; Bradford method; Lowry's method
93. Which of the following amino acids is encoded by six different codons?
(a) Alanine
(b) Glycine
(c) Serine
(d) Threonine
94. Which of the following molecules helps a protein to fold correctly in eukaryotes?
(a) GroEL
(b) Hsp90
(c) Protofoldins
(d) GroES

95. Which of the following configurations is not common to protein structures?
(a) Alpha helix
(b) Beta helix
(c) Alpha sheet
(d) Beta sheet
96. Polyalanine is a synthetic polypeptide whose concentration in aqueous solution could
be determined directly (i.e., without using any reagents) on a UV-visible
spectrophotometer at a wavelength of
(a) 220 nm
(b) 260 nm
(c) 280 nm
(d) 325 nm
97. Which of the following is not a glycosphingolipid?
(a) Cerebroside
(b) Ganglioside
(c) Globoside
(d) Sphingomyelin
98. When a compound with a chiral molecule is synthesized in the laboratory, it yields both
L- and D-forms. However, in living organisms only particular forms are present. The
form of molecules that does not exist in living organisms is
(a) D-glucose
(b) L-glucose
(c) L-amino acids
(d) D-amino acids
99. Which type of inhibition does the following example represent?
A-3B-31 C-3.D-- E
-- X-----
(a) Competitive inhibition
(b) Feedback inhibition
(c) Sequential inhibition
(d) Cumulative inhibition

100. In 2-D gel electrophoresis, the first dimension is based on the principle of
(a) isoelectric focusing
(b) high-voltage electrophoresis
(c) SDS-PAGE
(d) urea-PAGE
101. Match the following :
(A) Zero-order reaction (i)
(B) First-order reaction (ii)
(C) Second-order reaction (iii)
(D) Third-order reaction (iv)
(a)
(b)
(c)
(d)

102. If AG is negative
(a) the reaction is in equilibrium
(b) the reaction is spontaneous
(c) the reaction does not occur in forward direction
(d) the reaction may occur in forward or reverse direction
103. Which of the following is an incorrect statement?
(a) All proteins destined for the peroxisomal matrix bind to a cytosolic receptor
(b) I-cell disease is associated with impaired transport of proteins into the
peroxisomal matrix
(c) Peroxisomal targeting sequence may be C-terminal PTS1 or N-terminal PTS2
(d) Peroxisomal targeting sequence is non-cleavable

104. Which among the following would be the best technique to separate a protein that binds
strongly to its substrate analog? ,
(a) Gel filtration
(b) Cation-exchange chromatography
(c) Affinity chromatography
(d) Anion-exchange chromatography
105. Which of the following enzymes would be considered an exonuclease, an enzyme with
the ability to remove incorrectly incorporated nucleotides during replication?
(a) DNase-I
(b) Uridine N-glycosylase
(c) DNA polymerase
(d) dNTPase
106. Which of the following is/are responsible for degeneracy of a genetic code?
(a) First nucleotide of a codon
(b) Second nucleotide of a codon
(c) Third nucleotide of a codon
(d) Second and third nucleotides of a codon
107. The RNA polymerase core enzyme of a bacterium consists of all of the following, except
(a) a subunit
(b) p subunit
(c) S' subunit
(d) o factor
108. Which of the following can be used as a sequence similarity tool?
(a) PHYLIP
(b) PYMOL
(c) RASMOL
(d) BLAST

109. The composition of different nucleotides from DNA molecules of various organisms was
analyzed and the result is given below in the form of percent of different nucleotides :
Sample A C G T
1 35 15 15 35
2 20 30 30 20
3 28 24 24 28
4 35 20 30 15
Nucleotide composition of which of the following samples suggests that the DNA is a
single-stranded molecule?
(a) 1
(b) 2
(c) 3
(d) 4
110. Which of the following is a limitation for Hardy -Weinberg equilibrium?
(a) Lack of mutation
(b) One allele has selective advantage over the other
(c) No inbreeding
(d) Large population size
111. Which of the following molecules is dubbed as the second genetic code?
(a) rRNA
(b) Aminoacyl tRNA synthetase
(c) mRNA
(d) tRNA
112. Which of the following DNA sequences is likely to be the site for a restriction
endpnuclease?
(a) GTCGAC
(b) GTTCCA
(c) ATTTAA
(d) GCGGGC
113. Which of the following RNA species is least stable in a cell?
(a) mRNA
(b) tRNA
(c) rRNA
(d) snoRNA

114. Geeta has a paternal uncle with hemophilia B, an X-linked recessive disease. Her risk of
having a child with hemophilia B is best described as
(a) near 100%
(b) near 0%
(c) 50% for all male children
(d) 50% for all children
115. Colchicine-treated cells are arrested in
(a) S phase
(b) prophase
(c) G1 phase
(d) metaphase
116. - can be viewed as a 'reversible nuclease' that creates either a transient
single-strand break or a transient double-strand break.
(a) Si nuclease
(b) Topoisomerase
(c) DNA endonuclease
(d) RNase H
117. Which of the following is not true in Griffith's experiment?
(a) After coinjecting heat-killed smooth S. pneumoniae along with live rough
S. pneumoniae into a mouse , the mouse died
(b) Genetic material is heat and protease stable
(c) Genetic material does not get destroyed with the death of the cell
(d) Genetic material is sensitive to RNase treatment
118. In the preparation of an SDS-polyacrylamide, which of the following acts/act as the
polymerizing agent(s)?
(a) SDS
(b) TEMED and SDS
(c) Ammonium persulphate
(d) Bisacrylamide and ammonium persulphate
119. Which of the following is the correct order, from smallest to largest number of base
pairs?
(a) Plasmid, transposon, chromosomal DNA
(b) Chromosomal DNA, transposon, plasmid
(c) Transposon, plasmid, chromosomal DNA
(d) Plasmid, chromosomal DNA, transposon

120. Histocompatibility antigens in mouse and 'humans are encoded by
(a) H-3 complex in mice and HLA complex in humans
(b) HLA complex in mice and H-3 complex in humans
(c) H-2 complex in mice and HLA complex in humans
(d) HLA complex in mice and H-2 complex in humans
121. The half-life of 64Cu is 12.83 hours. How much time would it take to reach the
disintegration rate of 100 min-1 from 500 min-1?
(a) 0.054 hour (b) 0.13 hour
(c) 28.5 hours (d) 29.7 hours
122. de Broglie wavelength of an electron moving with a velocity of 5.3 x 106 m/s (mass of
electron = 9.1 x 10-31 kg and Planck 's constant = 6.6 x 10-34 J s) ,is
(a) 0.0137 A (b) 0.137 A
(c) 1-37 A (d) 13.7 A
123. A mixture contains 10 mL of 0.1 M disodium hydrogen phosphate and 20 mL of 0-1 M
monopotassium hydrogen phosphate. The final concentration of phosphate in the
solution is
(a) 0.33 M (b) 0.66 M
(c) 0.5 M (d) 0.1 M
124. In two different zero-order reactions A -4 P1 and B-> P2, the rate of conversion of A into
product is twice that of B converted into product . If 30% of B is converted into P2 in
30 minutes, then what will be- the amount of A reacted at the same time?
(a) 15% (b) 30%
(c) 45% (d) 60%
125. The number of nitrogen molecules in a one litre flask at 27 °C and 7-6 x 10-3 mm of Hg
pressure is
(a) 2.4x1017 (b) 2.4x1015
(c) 4.5x1017 (d) 4.5x1015
126. If 10 mL of a 1 M solution of barium hydroxide is diluted into 100 mL, then how many
millimoles of the compound would be present in the solution?
(a) 0.1 (b) 1
(c) 10 (d) 100

127, If 10 moles of ideal gas expand by two times in volume at atmospheric pressure and
300 K temperature , the change in entropy (in J/K) would be
(a) 57.63 (b) 5763
(c) 25.02 (d) 16628
128. A protein of molecular weight 55000 Da contains 020% Mn by mass. What is the
number of Mn atoms present in the protein?
(a) One (b) Two
(c) Three (d) Four
129. If hydrogen iodide decomposes at an activation energy of 290 kJ mol-1 at 307 °C, then
the fraction of molecules having energy equal to the activation energy would be
(a) e-60 (b) e60
(c) e-113 • (d) el 13
130. The pH of a buffer solution obtained by mixing equal volumes of 0.2 mole of hydrazine
and 0.1 mole of hydrazine hydrochloride in a final volume of 100 mL will be
(dissociation constant of hydrazine is 3 % 10-6 M)
(a) 5-22 (b) 5.82
(c) 8.78 (d) 8.18
131. The weight of oxygen that will react with 2 g of calcium to produce calcium oxide
(atomic weight of Ca = 40 and that of 0 = 16), considering complete conversion, is
(a) 5 g (b) 2 g
(c) 0-8 g (d) 0-5 g
132. At 25 °C, the specific conductance of a 0.01 M aqueous solution of acetic acid is
0.163 mS m-1. The molar conductance at infinite dilution is 390 x 10 -4 S m2 mo1-1.
What is the degree of dissociation of the acid?
(a) 0-017 (b) 00417
(c) 0-174 (d) 0-163

133. If the IR spectrum of a solution of substituted benzoic acid shows a strong H -bonding
vibrational transition which disappears upon dilution, then the compound could be
(a) ortho-hydroxybenzoic acid (b) ortho-methoxybenzoic acid
(c) para-hydroxybenzoic acid (d) para-methoxybenzoic acid
134. A gas expands by 11 L against an average pressure of 2 atm at 300 K. The work done by
the gas (in joules) is
(a) 2228.6 (b) 6600
(c) 1114-3 (d) 557-15
135. A current of 4.0 amperes deposits 0-8 g of sodium when passed through molten NaCl for
20 minutes . The electrochemical equivalent of sodium is
(a) 1.0 x 10-2 g/C (b) 2-s x 10-1 g/C
(c) 4-16 x 10-4 g/C (d) 1-67 x 10-4 g/C
136. How many grams of iodine are present in a solution which requires 20 mL of 0.11 N
Na2S2O3 to react with it , in the reaction S2O3 +I2 -*5406 +2I-?
(Molecular weight of iodine = 254)
(a) 2-54 g (b) 5-58 g
(c) 0.279 g (d) 12-7 g
137. Halogenated organic liquid insulators are
(a) silicones (b) silicates
(c) esters (d) Askarels
138. When hydrogen is used as fuel in a hydrogen -oxygen fuel cell, the electrode is made of
(a) an alloy of palladium and silver
(b) aluminum
(c) iron
(d) cadmium
139. . If the maximum concentration of PbCl2 in water is 0.01 Mat 25 °C, then its maximum
concentration in 0-1 M NaCl would be
(a) 2x103M
(b) 1x10-4 M
(c) 1.6 x 10-2 M
(d) 4x 10-4 M
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140. The optically active molecule from the following is
(a) C12CHCH3 (b) CH3CH(C2H5)OH
(c) CH3CH(CH3)OH (d) CH3CH(CH3)Cl
141. 20% cells in a mixed bacterial population are resistant to ampicillin. The cells are plated
and individual colonies (each representing a single cell) are checked for ampicillin
resistance. The probability that if 10 colonies are tested and none shows resistance to
antibiotic is approximately
(a) zero (b) 0.2
(c) 0.1 (d) 0.3
142. If the 2x2 matrix A=11 4] and 2A+3B=15 21, then B is equal to
-2
-1 2 1
(a) [ 1 -2] (b) [ 1 -2]
(c) 11 2] (d) [1 -2]
143. The values of a and b such that the function defined by
5, x<-2
f(x)= ax+b, 2<x<10
21, x?10
is continuous are
(a) 2 and 1 respectively (b) -2 and -1 respectively
(c) 1 and 2 respectively (d) -2 and 1 respectively
144. The differential equation x ^ - y = 0 represents
(a) a set of concentric circles
(b) a set of parabolas with vertex at (0, 0)
(c) a set of straight lines passing through the origin
(d) a set of parallel straight lines
/66-A 30
.. I Li Ill^1un,. 1 . L,qVyp I I MI , I, I il lp '!I
145. Let a, 0 be the roots of x 2 + ax + b = 0. Then a 2, 0 2 are the roots of
(a) x2+(2b-a2)x+b2=0
(c) x2 -a2x+b2 = 0
(b) x2+a2x+b2=0
(d) x2+2bx+b2=0
-146. If A is a 3x3 matrix and det (5A) = k(det(A)), then It is equal to
(a) 5 (b) 25.
(c) 125 (d) 625
147. The value of lim sin 3 x - x 3 is
X-,0 xcosx
(a) 0 (b) 1
(c) infinity (d) None of the above
148. The maximum value that the polynomial x3 - x can take in the interval 1-!Z 2) is
(a) - (b) 6
(c)
-2 (d) None of the above
149. The value of x such that the vectors 1 + j and 31 + xl are perpendicular is
(a) 9 (b) 1
(c) -1 (d) -3
150. A particle is moving in a circle of radius R with constant speed . The time period of the
particle is T. Ina time period from t = 0 to t = T / 6, it will have an average velocity of
(a) 2nR/T (b) 3R/T
(c) 6R/T (d) 2R/T
/66-A 31 1 P.T.O.
151. A ball of mass in is attached to one end of a very light rod of length 1, the other end of
which is hinged so that it can rotate freely . What minimum velocity v should be
imparted to the ball when it is at the bottommost position so that it can complete the
vertical circle?
(a) g1 (b) 5g1
(c) 3g1 (d) 2g1
152. The magnitude of the potential energy per unit mass of the object at the surface of the
earth is E. Then the escape velocity of the object is
(a) JE (b) 2E
(c) 4E2 (d) E/2
153. The speed of transverse wave in a string of density 100 kg/m3 and area of cross-section
10 mm2 under a tension of 103 N is
(a) 100 m/s (b) 1000 m/s
(c) 200 m/s (d) 2000 m/s
154. String 1 has twice the length , twice the thickness, twice the tension and twice the
density of another string 2. The relation between the fundamental frequency as of 1 and
2 is
(a) fl = 2f2 (b) fl = 4f2
(c) f2 = 2f1 (d) fl = f2
155. If 2 moles of an ideal mono -atomic gas at temperature TO are mixed with 4 moles of
another ideal mono-atomic gas at temperature 2T0, then the temperature of the mixture
is
(a) 3 TO (b) 2 TO
4 5
(c) 3 TO (d) 4 TO
156. A gas at pressure PO is contained in a vessel . If the masses of all the molecules are
halved and their speeds doubled , the resulting pressure would be
(a) 4P0 (b) 2P0
(c) Po (d) PO /2

157. From a concave mirror of focal length f, image (assume virtual) is 2 times larger. Then
the object distance from the mirror is
(a) f /2
(b) 3f /2
(c) f /4
(d) 4f /3
158. In an Atwood machine, the two masses are 450 g and 550 g. If the acceleration due to
gravity is g= 10 m/s2, the acceleration of the masses is
(a) 9.8 m/s2
(b) 10 m/s2
(c) 1.0 m/s2
(d) 0.5 m/s2
159. Carrot engine A operates between the temperatures of TH = 600 K and TL = 300 K,
while Carrot engine B between the temperatures of TH = 500 K and TL = 200 K. If the
efficiencies of the two engines are 11A and tIB, which of the following statements is true?
(a)
(b)
(c)
(d)
'IA >IIB
T1A <1B
T1A =1B
11A may be greater or less than 11B depending on the nature of the working
substance used in the two engines
160. Three corners of a square have equal charges +1 C on them. The electric field at the
centre will be
(a) zero
(b) along the diagonal and towards the empty comer
(c) along the diagonal and away from the empty corner
(d) parallel to one of the sides