Bharathiar University Biotechnology Syllabus

My bother having completed 12th with Science stream, now wants to do B.Sc. Biotechnology from Bharathiar University, so will you please provide here Bharathiar Univ B.Sc. Biotechnology syllabus to get idea about course outline???

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You are looking for Bharathiar University B.Sc. Biotechnology syllabus to get idea about course outline, here I am giving:

B.Sc. Biotechnology

Semester I
Language – I
English – I
Core Paper I - Cell biology
Core Paper II - Biodiversity
Core practical I
Allied A : Chemistry I
Allied Practical
Environmental Studies

Semester II
Language – II
English – II
Core Paper III - Biochemistry
Core Practical I (cell Biology & biochemistry)
Allied A : Chemistry II
Allied Practical (Chemistry)
Value Education – Human Rights

Semester III
Language – III
English – III
Core Paper IV - Microbiology
Core Paper V Genetics
Core Practical II
Allied B: Paper I – Basic Mathematics
Skill based Subject I – Diploma Paper I -
Human Physiology
BU B.Sc Biotech syllabus
CORE PAPER: I
Subject Title: CELL BIOLOGY
Subject description: This course presents the types and structural details of the basic
unit by which all the living things are made of (the cell).
Goals: To make the student to understand the concept of cell and their activities.
Objectives: On successful completion the subject student should have understand:
Structural features, Organelles and the cellular mechanisms.
UNIT I
Cell as a basic unit: discovery of the cells, classification of cell types,
development of cell theory, early chemical investigation in cell biology. Prokaryotic and
Eukaryotic cell organization.
UNIT II
Cell transport phenomenon: membrane architecture. Active, Passive, diffusion
and osmosis. Chemistry of carbohydrates, lipids, proteins and nucleic acids.
UNIT III
Structure and function of cytoplasmic compartments of the cell: ribosome and
protein synthesis, energy flow through mitochondrion, chloroplast and photosynthesis,
Golgi apparatus, lysozymes and micro bodies, endoplasmic reticulum , cytoskeleton,
vacuoles, peroxysomes, lysozomes and Nuclear compartment. Heterochromatin and
euchromatin, polytene chromosomes.
UNIT IV
Cell division in prokaryotes and eukaryotes: Cell cycle, mitosis, meiosis, crossing
over and characteristics of cancer. Apoptosis, Stem cell. Prions.
UNIT V
Integrative and specialized cellular events, cell-cell signaling, specialized cells
nerve cells, sperm cells, microfilaments, microtubules, muscle cells. Cells of vision,
Nucleo-cytoplasmic interaction, cell cloning.
References
1. Cell and molecular biology, 3rd edition, Philip Sheeler, Donal E Bianchi, John
Wiley
2. Molecular biology of cell, Alberts et al
3. Molecular cell biology, Lodish, Baltimore, Scientific American books, 1994
4. Molecular and cell biology, Stephen L Wolfe, Wordsworth Publishing company
1993
5. Cell biology. Sadava
6. Cell and Molecular Biology De Roberties
CORE PAPER: II
Subject Title: BIODIVERSITY
Subject description: This course presents the Divers nature of the organisms
Goals: To make the student to understand the diversity of the nature
Objectives: On successful completion the subject student should have understand:
Species and genius on the earth, conservation and development of biological resources.
UNIT-I
General aspects of biodiversity-General introduction. Types of Biodiversity-
Global biodiversity, biodiversity in India-Species biodiversity, Measures of biodiversity-
Loss of biodiversity. Listing of threatened biodiversity-threatened animals, Plants, causes
for the loss of biodiversity. Biodiversity-strategy and action plan.
UNIT II
Species concept; biological nomenclature. Theories of biological classification;
structural, biochemical and molecular systematic. DNA fingerprinting, numerical
taxonomy, magnitude and distribution of biodiversity, economic value, wild life biology,.
UNIT III
Broad outlines of classification and evolutionary trends among algae, fungi,
bryophytes and terydophytes. Economic importance of algae, fungi and lichens. Biotic
community-concept, structure, dominance, fluctuation and succession; N,P,C,S cycles in
nature Principles of conservation, conservation strategy and sustainable development.
UNIT IV
Interaction between environment and biota; concept of habitat and ecological
niches, limiting factor, energy flow, food chain, food web and tropic levels, ecological
pyramids and recycling.. Ecosystem dynamics and management, stability and complexity
of ecosystem, speciation and extinctions, environmental impact assessment.
UNIT V
Physiochemical properties of water, distribution and impact of experimental
factors on the aquatic biota, productivity, mineral cycles and biodegradation in different
ecosystem, biology and ecology of reservoirs.
References
1. Glimpses of Biodiversity- B.Blosetti.
2. Environmental biodiversity- P.R.Yadav
3. Biodiversity of microbial life- Stanely Reysenbach
4. Ecology & Env. Biology Sathyanarayana Books & Allied (P) Ltd
CORE PAPER: III
Subject title: BIOCHEMISTRY
Subject description: This course presents the chemical reactions or metabolic functions
in the living system and their regulations.
Goals: To make the student to understand the concept of biochemical regulations
Objectives: On successful completion the subject student should have understand:
Basic metabolism
Enzymes and their kinetics
Applications of metabolites
UNIT I
Structure of atoms and biomolecules: atomic theory, valancy, atomic weight, molecular
weight, Molarity.
Chemical Bonding, properties of Water ionization pH and buffers
UNIT II
Introduction to Biochemistry: Fundamental Structures of proteins, nucleic acid, lipid and
carbohydrates.
Thermodynamics in biology: Energy metabolism. Free energy, energy rich bonds, week
interactions, coupled reactions, kinetics, association dissociation concepts, biological
energy transducers.
UNIT III
Enzymes and co-enzymes, IUB classification and nomenclature of enzymes, enzyme
kinetics (positive and negative cooperativity), regulation of enzyme activity, active sites,
activators and inhibitors; allosteric enzymes
UNIT IV
Classifications and reactions of sugars. structural features of polysaccharides. Glycolysis,
TCA cycle, glycogen breakdown and synthesis, gluconeogenesis, bioconversion of
pentoses and hexoses, ETC, Oxidative and photo phospharylation
Classification and functions of lipids.
Metabolism of lipids; Fatty acid biosynthesis and oxidations,
UNIT V
Amino acids and peptides – classifications chemical reactions and physical properties,
metabolisms of amino acids. Functions of proteins.Biosynthesis and degradation of
nucleic acids (purines and pyrimidines) Integration of metabolism and regulations.
Secondary metabolites –applications,
isoprenoids and hetero cyclic compounds
REFERENCES:
B.Sc. Biotechnolog (Colleges) 2008-09 Annexure No. 37 A
Page 6 of 25 SCAA Dt. 21.05.2009
1. Boyer.R., (2002) Concepts in Biochemistry 2nd ed. Brooks/cole publishing company
New York.
2. David L. Nelson and M. Cox (2003) Lehninger’s Principles of Biochemistry, 3rd Ed,
Worth publication New York
3. Voet & Voet (1995) Fundamentals of Biochemistry, 2nd Ed, John Wiley and sons inc.,
New York.
4. Geoffery L Zubay (1995) Principles of Biochemistry, WCB publishers, London
5. Murrey RK., D.K. Granner, P.A. Mayers and V.W. Rodwell, (2003) Harper’s
Biochemistry, Prentice –Hall Int, Boston
6. Outlines of Biochemistry Conn & Stumph
7. Biochemistry 3rd ed Sathyanarayana Books & Allied (P) Ltd
CORE PRACTICAL I:
(CELL BIOLOGY AND BIOCHEMISTRY)
1. Microscopy
2. Cell Types --- Microbial, animal and Plant cells – simple staining and visualization
through microscope
3. Fraction of Cellular components—Demonstration.
4. Mitotic Preparation – Onion Root Tip, Grasshopper Hepatic coacae
5. Meiotic Preparation from Grasshopper Testis and flower buds of Rheo discolor
6. Cell Staining – Cytochemical Methods for Demonstration of Cellular and sub-cellular
components.
7. Estimation of Protein – Lowry’s, Bradford’s method.
8. Estimation of DNA--- DPA Method.
9. Estimation of RNA by Orcinol method
10. Estimation Of Sugars ---Anthrone and Benedict method
11. Estimation of total free amino acids --- Sulfovanicillin method.
12. Estimation of Lipids.
13. Analysis of Oils—Iodine Number---Saponification Value ---Acid Number.
14. Quantification of Vitamin C.
15. Thin Layer Chromatography.
16. Paper Chromatography.
17. Isolation of Enzymes.
18. Enzyme assay.
19. Determination of Km value.
CORE PAPER: IV
Subject Title: MICROBIOLOGY
Subject description: This course presents the study of Micro organisms.
Goals: To make the student to understand Micro organisms and their participation in day
to day activities.
Objectives: On successful completion the subject student should have understand:
What are micro organisms?
Their studying methods and their positive and negative on our lives
UNIT I:
Definition and scope of microbiology-- A general account on microbial diversity.Basic
principles in microscopy, Types of microscopes- light, dark, phase contrast, fluorescent
and electron microscope- (Transmission and Scanning electron)
UNIT II:
A detailed account of General structure, growth and reproduction of the various Bacteria,
fungi and Viruses. Economic and industrial importance of yeast and moulds
UNIT III:
Microbiological Media: Types, preparation, methods of sterilization; enumeration of
microorganisms in soil, water and air; isolation of microorganisms from Environment and
infected tissue; Techniques of pure culture, maintenance and Preservation; Staining:
stains and types of staining;
UNIT IV:
Physiology and biochemistry of microbes--Nutrition (Photo-autotrophs, Chemoautotrophs,
Parasitism, Saprophytism, Mutualism and Symbiosis, Commensalisms,
endozoic microbes) -- microbial pathogens of plants, animals and Humans.
UNIT V:
Respiration and fermentation, Nitrogen metabolism including Nitrogen fixation
(Symbiotic and asymbiotic), Lipid metabolism, Secondary metabolism, Production of
enzymes and antibiotics--Role of microbes in biogeochemical cycles.
References:
1. Michael T. Madigan John M. Martin & Jack Parker, 1984, Biology of
Microorganisms Prentice Hall International, Inc., London.
2. Edward A. Birge, 1992, Modern Microbiology – Principles and application.
Wm.C. Brown Publishers, Inc. U.S.A.
3. Gerard J. Tortora, Berdell R. Funke, Christine & L. Case, 2001, Microbiology -
An Introduction. Benjamin Cummings, U.S.A.
4. Danial Lim, 1998, Microbiology, McGraw-Hill Companies, New York.
5. Stephen A. Hill, 1984, Methods in Virology. Blackwell Scientific Publication, London.

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