Bangalore University DNA

Is this Bangalore University offers DNA Science Online Course (CEBI0935)? If yes then will you please tell me from where I can download the syllabus of this course? also give me the procedure to download and print the offline application form to apply for the DNA Science Online Course (CEBI0935) of the Bangalore University?

Hii sir, I Wants to get the Syllabus of the GNT 501: Recombinant DNA Technology) V Sem of BSc Genetics of the Bangalore University ?

[pooja]

Bangalore University, or BU, is a public state university is located in Bengaluru in the state of Karnataka, India. The university is a part of the Association of Indian Universities (AIU), Association of Commonwealth Universities (ACU) and affiliated by University Grants Commission (UGC).

The Syllabus of the GNT 501: Recombinant DNA Technology) V Sem of BSc Genetics of the Bangalore University is given below


GNT 501: Recombinant DNA Technology

UNIT I
a. Introduction to RDT:
Overview of major steps involved
b. Tools for RDT:
# Enzymes:
 Restriction endonucleases: Types and characteristic features; Nomenclature;
Modification of cut ends
 DNA ligases
 Other enzymes:A brief account of Alkaline phosphatase,
Polynucleotide kinase, Exonuclease III, DNase I,
DNA polymerase and Klenow fragment,
Terminal nucleotidyl transferase, RNA dependent DNA
Polymerase.
# Vectors:
 Properties of an ideal vector
 Types : Cloning and expression vectors

i) Cloning vectors: i) Prokaryotic vectors: Plasmids- pBR 322; pUC 18;
Bacteriophages- Lambda phage, Cosmids.
ii) Eukaryotic vectors: YAC vectors; Shuttle vectors- Yeast and E. coli.
iii) For higher plants: Integrative DNA transfer- Agrobacterium vectors-
Ti plasmid-Binary and Co integrated vectors; Non integrative
DNA transfer-Plant viral vectors (CaMV)
iv) For animals: Animal viral vectors- SV 40 (3 types);
ii) Expression vectors in Prokaryotes and Eukaryotes


UNIT II
a. Isolation of the desired gene:
# cDNA library,
# Genomic library,
# Organo-chemical synthesis,
# Amplification through PCR

Contact Details
Bangalore University
Reception / Enquiry, Jnanbharathi Campus: 080-22961000