#1
18th July 2015, 08:42 AM
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Aligarh Muslim University Msc Biotechnology
Hello sir I want to do Msc in Biotechnology from Aligarh Muslim University so would you please tell me does Aligarh Muslim University offer Msc in Biotechnology if yes then please provide me details about this…
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#2
18th July 2015, 03:33 PM
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Re: Aligarh Muslim University Msc Biotechnology
Aligarh Muslim University (AMU) was established in 1875, it is a public university, funded by the central government of India Yes dude, Aligarh Muslim University offer Msc in Biotechnology Duration: 2 year Eligibility: Candidates must have passed Bachelor of Science (BSc) in Biotechnology from recognized university…. Number of Seats : 14 Syllabus: SEMESTER I Biochemistry 3 Credits Unit I Chemical basis of life; Composition of living matter; Water – properties; pH; ionization and hydrophobicity; Emergent properties of biomolecules in water; Biomolecular hierarchy; Macromolecules; Molecular assemblies; Structure-function relationships Amino acids – structure and functional group properties; Peptides and covalent structure of proteins; Elucidation of primary and higher order structures; Evolution of protein structure; Structure-function relationships in model proteins like ribonuclease A; myoglobin; hemoglobin; chymotrypsin etc.; Tools to characterize expressed proteins. Unit II Enzyme catalysis – general principles of catalysis; Quantitation of enzyme activity and efficiency; Enzyme characterization and Michaelis-Menten kinetics; Relevance of enzymes in metabolic regulation; activation; inhibition and covalent modification; Single substrate enzymes Unit III Sugars - mono; di; and polysaccharides; Suitability in the context of their different functions- cellular structure; energy storage; signaling; Glycosylation of other biomolecules - glycoproteins and glycolipids; Lipids - structure and properties of important members of storage and membrane lipids; lipoproteins Unit IV Biomembrane organization - sidedness and function; Membrane bound proteins - structure; properties and function; Transport phenomena; Nucleosides; nucleotides; nucleic acids - structure; diversity and function; sequencing; Brief overview of central dogma. Unit V Bioenergetics-basic principles; Equilibria and concept of free energy; Coupled processes; Glycolytic pathway; Kreb's cycle; Oxidative phosphorylation; Photosynthesis; Elucidation of metabolic pathways; Logic and integration of central metabolism; entry/ exit of various biomolecules from central pathways; Principles of metabolic regulation; Regulatory steps; Signals and second messengers. Texts/References: 1. V.Voet and J.G.Voet; Biochemistry; 3rd edition; John Wiley; New York; 2004. 2. A.L. Lehninger; Principles of Biochemistry; 5th edition; W.H Freeman and Company; 2004. 3. L. Stryer; Biochemistry; 5th edition; W.H. Freeman and Company; 2002. Analytical Techniques 3 Credits Unit I Basic Techniques Buffers; Methods of cell disintegration; Enzyme assays and controls; Detergents and membrane proteins; Dialysis; Ultrafiltration and other membrane techniques Spectroscopy Techniques UV; Visible and Raman Spectroscopy; Theory and application of Circular Dichroism; Fluorescence; MS; NMR; PMR; ESR and Plasma Emission spectroscopy Unit II Chromatography Techniques TLC and Paper chromatography; Chromatographic methods for macromolecule separation -Gel permeation; Ion exchange; Hydrophobic; Reverse-phase and Affinity chromatography; HPLC and FPLC; Criteria of protein purity Electrophoretic techniques Theory and application of Polyacrylamide and Agarose gel electrophoresis; Capillary electrophoresis; 2D Electrophoresis; Disc gel electrophoresis; Gradient electrophoresis; Pulsed field gel electrophoresis Unit III Centrifugation Basic principles; Mathematics & theory (RCF; Sedimentation coefficient etc); Types of centrifuge - Microcentrifuge; High speed & Ultracentrifuges; Preparative centrifugation; Differential & density gradient centrifugation; Applications (Isolation of cell components); Analytical centrifugation; Determination of molecular weight by sedimentation velocity & sedimentation equilibrium methods Unit IV Radioactivity Radioactive & stable isotopes; Pattern and rate of radioactive decay; Units of radioactivity; Measurement of radioactivity; Geiger-Muller counter; Solid & Liquid scintillation counters (Basic principle; instrumentation & technique); Brief idea of radiation dosimetry; Cerenkov radiation; Autoradiography; Measurement of stable isotopes; Falling drop method; Applications of isotopes in biochemistry; Radiotracer techniques; Distribution studies; Isotope dilution technique; Metabolic studies; Clinical application; Radioimmunoassay Unit V Advanced Techniques: Protein crystallization; Theory and methods; API-electrospray and MADI-TOF; Mass spectrometry; Enzyme and cell immobilization techniques; DNA & Peptide Synthesis. Texts/References: 1. Freifelder D.; Physical Biochemistry; Application to Biochemistry and Molecular Biology; 2nd Edition; W.H. Freeman & Company; San Fransisco; 1982. 2. Keith Wilson and John Walker; Principles and Techniques of Practical Biochemistry; 5th Edition; Cambridge University Press; 2000. 3. D. Holme & H. Peck; Analytical Biochemistry; 3rd Edition; Longman; 1998. 4. R. Scopes; Protein Purification - Principles & Practices; 3rd Edition; Springer Verlag; 1994. 5. Selected readings from Methods in Enzymology; Academic Press. Biostatistics & Computer Applications 3 Credits Unit I Fundamental concepts in applied probability; Exploratory data analysis and statistical inference; Probability and analysis of one and two way samples; discrete and continuous probability models; Expectation and variance; Central limit theorem; Inference; Hypothesis; Critical region and error probabilities; Tests for proportion; Equality of proportions; equality of means of normal populations(variance known; variance unknown); Chi-square test for independence; P-value of the statistic; Confidence limits; Introduction to one way and two-way analysis of variance; Data transformations Unit II Elements of programming languages - C and PERL; Data base concept; Database management system; Database browsing and Data retrieval; Sequence database and genome database; Data Structures and Databases; Databases such as GeneBank; EMBL; DDBJ; Swissplot; PIR; MIPS; TIGR; Hovergen; TAIR; PlasmoDB; ECDC; Searching for sequence database like FASTA and Blast algorithm. Unit III Cluster analysis; Phylogenetic clustering by simple matching coefficients; Sequence Comparison; Sequence pattern; Regular expression based pattern; Theory of profiles and their use in sequence analysis; Markov models; Concept of HMMS; Baum-Welch algorithm; Use of profile HMM for protein family classification; Pattern recognition methods Unit IV Goals of a Microarray experiment; Normalization of Miroarray data; Detecting differential gene expression; Principle component analysis; Clustering of microarray data; Structure determination by X-ray crystallography; NMR spectroscopy; PDB(Protein Data Bank) and NDB(Nucleic Acid Data Bank); File formats for storage and dissemination of molecular structure. Unit V Methods for modeling; Homology modeling; Threading and protein structure prediction; Structure-structure comparison of macromolecules with reference to proteins; Force fields; Molecular energy minimization; Monte Carlo and molecular dynamics simulation Practicals: Introduction to MSEXCEL-Use of worksheet to enter data; edit data; copy data; move data. Use of in-built statistical functions for computations of Mean; S.D.; Correlation; regression coefficients etc. Use of bar diagram; histogram; scatter plots; etc. graphical tools in EXCEL for presentation of data. Introduction to SYSTAT package. Searching PubMed ; Introduction to NCBI; NCBI data bases; BLAST BLASTn; BLASTp; PSIBLAST; Sequence manipulation Suite; Multiple sequence alignment; Primer designing; Phylogenetic Analysis. Protein Modeling; Protein structure Analysis; Docking; Ligplot interactions. Contact detail: Aligarh Muslim University Aligarh, Uttar Pradesh Map; [MAP]Aligarh Muslim University[/MAP] Rest of the syllabus here I am attaching pdf file which is free for download… |